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Table 2 Natural scrapie isolates analysed with discriminatory western blotting by using mAbs P4 and SAF842.

From: PRNP genetic variability and molecular typing of natural goat scrapie isolates in a high number of infected flocks

Sample's ID

PRNP genotype

Target group

Age (months)

rel ratio

SD

mw

SD

dig

SD

mono

SD

non

SD

GR-L1-G1

wt, 240P/P

sl

21

1.21

0.35

17.54

0.17

50

2

31

1

19

3

GR-D1-G1

wt, 240P/P

sl

86

0.63

0.19

17.70

0.02

52

1

32

1

16

2

GR-E1-G1

wt, 168Q/Q, 240P/P

sl

40

          

GR-B1-G1

wt, 240P/S

sl

58

1.15

0.41

17.54

0.08

53

2

33

5

14

4

GR-M1-G1

no amplicon

sl

43

1.21

0.21

17.60

0.17

53

1

30

3

17

4

GR-N1-G1

no amplicon

sl

20

1.05

0.09

17.57

0.16

52

2

32

2

17

3

GR-F1-G1

wt, 240P/P

sl

75

0.97

0.19

17.67

0.10

52

1

30

2

18

4

GR-A9-G1

wt, 240P/P

sl

48

1.11

0.12

17.62

0.11

54

1

30

3

16

4

GR-A4-G1

wt, 240P/S

sl

36

1.02

0.28

17.59

0.02

55

1

29

2

16

3

GR-A4-G2

wt, 240P/P

sl

36

0.93

0.22

17.59

0.12

54

2

30

3

16

4

GR-A4-G3

wt, 240P/P

sl

36

0.84

0.09

17.62

0.12

51

3

31

3

17

6

GR-A4-G4

wt, 240P/P

sl

24

0.85

0.16

17.61

0.12

52

2

30

2

18

3

GR-A4-G5

wt, 240P/P

susp. sl

48

1.10

0.07

17.58

0.11

50

2

31

2

18

3

GR-A4-S1

wt

sl

-

1.01

0.13

17.59

0.10

49

2

34

3

16

4

GR-A11-G1

wt, 240P/P

susp. flock

24

0.61

0.12

17.72

0.03

52

2

32

2

16

5

GR-A11-G2

wt, 240P/S

susp. flock

26

0.58

0.08

17.82

0.10

53

4

29

3

18

4

GR-A11-S1

wt

susp. flock

9

0.41

0.01

17.96

0.10

48

2

31

0

21

1

GR-A11-S2

ARQ/TRQ

susp. flock

9

0.41

0.02

17.92

0.06

48

4

32

1

20

3

GR-A2-G1

wt, 222Q/K, 240S/S

sl

36

0.38

0.01

18.03

0.07

48

2

35

1

17

1

GR-A2-G2

wt, 222Q/K, 240S/P

sl

36

0.50

0.03

18.04

0.02

48

2

33

2

19

4

IT1-goat

wt, 240P/S

-

-

0.98

0.05

17.56

0.11

48

2

31

3

20

3

IT2-goat

wt, 240P/S

-

-

0.90

0.03

17.52

0.07

49

2

34

3

17

4

IT3-goat

wt, 240P/S

-

-

0.87

0.17

17.51

0,04

53

3

30

2

17

1

IT4-goat

wt, 240P/P

-

-

1.05

0.07

17.56

0.06

52

4

29

1

19

3

IT5-sheep

wt

-

-

1.00

 

17.59

0.07

52

3

31

2

17

3

sheep BSE

wt

exp

-

8.58

1.85

16.78

0.07

71

4

20

2

9

3

  1. 2 Sample's ID indicates the country of origin (GR for Greece, IT for Italy), the geographic area of origin (alphabetical letters), the flock from that area (i.e. A1) and the number of the goat (G) or sheep (S, in italics)-cases from the same flock (G1), i.e. GR-A1-G1. Abbreviations: wt: ARQ/ARQ wild type, sl: slaughtered, susp.: TSE suspect in, exp: experimentally infected, rel ratio: relative ratio, SD: standard deviation, mw: molecular weight, dig: di-glycosylated PrPres, mono: mono-glycosylated PrPres, non: non-glycosylated PrPres.