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Table 1 Experiment 2: Cell numbers (mean ± SEM) and apoptotic cell ratios (mean ± SEM) of blastocysts in different treatment groups at 48 and 72 hours post exposure (hpe)

From: Susceptibility of in vitro produced hatched bovine blastocysts to infection with bluetongue virus serotype 8

Group and treatment

Total cell number (mean ± SEM)

Apoptotic cell ratio (mean ± SEM)

 

at 48 hpe

at 48 hpe

SOF (n = 6)

213.3 ± 23.12

14.5 ± 0.98a

MEM (n = 5)

312.8 ± 100.97

7.0 ± 0.64b

BTV 103.8 (n = 7)

107.1 ± 21.47

18.9 ± 1.43c

BTV 104.9 (n = 4)

108.4 ± 18.72

11.8 ± 1.55a

 

at 72 hpe

at 72 hpe

SOF (n = 5)

413.1 ± 119.61

3.9 ± 0.43a

MEM (n = 7)

331.7 ± 83.24

4.7 ± 0.44a

BTV 103.8 (n = 5)

129.3 ± 25.25

14.9 ± 1.29b

BTV 104.9 (n = 3)

105.7 ± 23.31

16.6 ± 2.6b

  1. Mock-exposed, control blastocysts (SOF and MEM) were incubated in BTV-8 free SOF culture medium and MEM medium respectively. Virus exposed blastocysts were exposed to two different titers of BTV-8 i.e. BTV 103.8 or BTV 104.9 TCID50 respectively.
  2. abc Values with different superscript within the same time point and column differ significantly.