Experimental infection of Pacific oyster Crassostrea gigas spat by ostreid herpesvirus 1: demonstration of oyster spat susceptibility

Table 1 Description of the four different initial tissue homogenates made from naturally OsHV-1 infected oyster spat collected on the field in France during the abnormal mortality outbreak in 2008

Name	Origin of animals	Virus DNA amount	OsHV-1 genotype
Homogenate 1	Normandy (Cotentin)	4.21 × 10²	μVar
Homogenate 2	South Brittany (Morbilhan)	2.18 × 10³	μVar
Homogenate 3	North Brittany (Bay of Brest)	2.03 × 10²	μVar
Homogenate 4	Mediterranean sea	0	-

Virus DNA amounts of oyster tissue homogenates were quantified by real-time quantitative PCR and expressed as the number of virus DNA copies per μL of water. OsHV-1 genotypes of homogenates were determined by sequencing of the virus genome.

ISSN: 1297-9716