Table 1 Oligonucleotide sequences used in the PCRs performed in this study and amplicon length.
From: Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection
PCR | Oligonucleotides 5'- 3' | Amplicon length |
|---|---|---|
MR0 | MR0Fw CCATGAGGCTACCCCTGCTCCTGGTT MR1Rv GCGTACCACTTGTTTTCAAACTTG | 560 nt |
MR1 | MR1Fw CCGAATCTCAGATTATGAGTGTTGC MR2Rv CTTGCAGATGTAGCCAAGAGGCC | 1253 nt |
MR2 | MR6Fw GGCAAAGATGGATACTGGGCAG CDR5Rv CATTTGCAAAATTGGGTTCACC | 1279 nt |
MR3 | CDR4Fw GGCGAACCTAATAATTATCAG MR7Rv GTGCATCCAGGCAAAAGCATTAC | 1210 nt |
MR4 | MR10Fw ACAGGTGATCCCTCTGGTGAAAGA MR10Rv CTAGATGRCCRCATGTTCRTTCTG | 408 nt |
qMR (CDR4-CDR5) | MR Fw TGGCAAATCCAGTTGTTAAGATGTT MR Rv AGAATGTTGAATACTGTGGCGAGTT | 91 nt |
β-actin (control) | Actin 663 Fw CTCACGGAGCGTGGCTACA Actin 769 Rv GCCATCTCCTGCTCGAAGTC | 106 nt |
qPCR (p17 EV1) | MVV0262Fw CTCCTTGCAGGCCACAATG MVV0333Rv GCTGCTTGCACTGTCTCGG MVV0284P 6-FAM-TGCCTTATGTGTAGTCAGC-TAMRA | 71 nt |