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Figure 4 | Veterinary Research

Figure 4

From: Recovery of infectious virus from full-length cowpox virus (CPXV) DNA cloned as a bacterial artificial chromosome (BAC)

Figure 4

Restriction fragment analysis of of pBR and pBRf. A. BAC DNA was digested with SphI, SacI or XhoI and fragments separated by 0.8% agarose gel electrophoresis. Changes of the restriction pattern due to the inserted TK duplication are marked with a box. Sizes of a molecular weight marker (generuler 1-kb plus DNA ladder, Fermentas) are given. B. Southern blot analysis after transfer of the gel shown in (A) and using a TK DIG labeled probe. The expected sizes of the fragments were identified (see text). Sizes of a molecular weight marker (generuler 1-kb plus DNA ladder, Fermentas) are given.

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Veterinary Research

ISSN: 1297-9716