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Table 2 Proteins identified in this study.

From: Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis

Description1

Spots2

O11 CDS3

O46 CDS3

ED133 CDS3

pI4

Mass (Da)5

Score6

Cov.7

#pep.8

EmPAI9

Comp.10

Loc.11

Immun.12

Metabolism

             

Energy production and conversion

             

formate acetyltransferase

81, 83, 82

011_0041

046_0511

SAOV_0163

5,31

84808

1301,60

34,31

22

1,57

CW

C

 

ldh L-lactate dehydrogenase

82, 99, 81, 83, 95

011_0136

046_0198

SAOV_2646c

4,80

34389

1749,37

63,64

26

21,75

CW

C

[82]

L-LACTATE DEHYDROGENASE (O11)

64

011_0021

046_0531

SAOV_0178

5,00

34548

745,83

50,79

14

2,59

S

C

[82]

bifunctional acetaldehyde-CoA/alcohol dehydrogenase

79

011_0796

046_0709

SAOV_0095

5,68

94945

2090,64

45,45

33

2,26

CW

C

 

D-3-phosphoglycerate dehydrogenase

100

011_0585

046_1131

SAOV_2344

5,32

34652

717,85

43,53

11

1,72

CW

C

 

atpD F0F1 ATP synthase subunit beta

62, 90

011_2064

046_0898

SAOV_2144c

4,68

51368

631,03

30,21

10

0,98

S,CW

C

[83]

Nucleotide transport and metabolism

             

adk adenylate kinase

9

011_0510

046_1206

SAOV_2266c

4,80

23375

531,72

42,38

9

2,80

S

C

[84]

deoD purine nucleoside phosphorylase

9

011_1051

046_0577

SAOV_2178

4,85

25892

470,94

53,39

8

1,97

S

C

[4]

guaB inositol-monophosphate dehydrogenase

102, 129

011_0077

046_0960

SAOV_0412

5,61

52818

1237,95

54,30

20

3,52

CW,T

C

[4]

Carbohydrate transport and metabolism

             

gpmA 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase

131

011_1952

046_0264

SAOV_2463c

5,23

26663

271.65

30.26

5

0.80

S,T

C

 

eno enolase 2-phosphoglycerate dehydratase

62, 88, 90, 96, 137

011_2336

046_2241

SAOV_0818

4,55

47088

1868,52

63,59

25

7,09

S,CW,T

C

[4, 52, 85–87]

fda fructose-1,6-bisphosphate aldolase

107, 94, 72, 91, 95

011_0131

046_0202

SAOV_2650

5,06

32878

852,09

46,62

13

2,48

CW,S

C

[85–88]

gap glyceraldehyde-3-phosphate dehydrogenase

99, 57, 81, 82, 83

011_2340

046_2237

SAOV_0814

4,89

36258

940,06

46,13

13

3,04

CW,S

C

[82, 86, 87, 89, 90]

putative translaldolase

9

011_1872

046_2451

SAOV_1765

4,72

25689

554,11

46,84

10

3,32

S

C

 

tpiA triosephosphate isomerase

9

011_2338

046_2239

SAOV_0816

4,81

27271

1396,16

76,28

21

16,78

S

C

[4, 91, 92]

atl autolysin

23, 31

011_1991

046_0320

SAOV_0999c

9,59

136983

2567,68

42,14

42

1,87

S

S

[52, 76, 93]

pyk pyruvate kinase

105

011_1282

046_0580

SAOV_1685

5,23

63067

271,45

11,62

5

0,29

CW

C

[94]

Lipid metabolism

             

fabZ 3R-hydroxymyristoyl ACP dehydratase

126

011_2356

046_1839

SAOV_2140c

5,71

16071

86,65

13,70

2

0,47

T

C

 

acetoin reductase

106

011_1332

046_1393

SAOV_0074

5,04

27199

566,84

40,31

7

1,82

CW

C

 

Amino acid transport and metabolism

             

dipeptidase PepV

62

011_1904

046_1329

SAOV_1737

4,56

52762

235.33

10.66

4

0,27

S

C

 

CYSTEINE SYNTHASE (O11)

64

011_1382

046_2402

SAOV_0535

5,38

32955

266.80

28.71

5

0,61

S

C

 

Information storage and processing

             

Translation, ribosomal structure and biogenesis

             

fus elongation factor G

95

011_0401

046_1769

SAOV_0582

4,80

76564

2173,77

68,11

30

4,11

CW

C

[3, 90]

prs 50S ribosomal protein L25/general stress protein Ctc

94

011_1370

046_2414

SAOV_0523

4,39

23773

384,17

34,56

6

2,26

CW,S

C

[95]

rplC 50S ribosomal protein L3

131

011_0531

046_1185

SAOV_2287c

9,72

22648

482,78

42,11

9

2,95

T

C

[95]

rpsA 30S ribosomal protein S1

137, 62, 121, 171

011_2142

046_1743

SAOV_1482

4,51

43252

1555,46

71,36

23

6,25

S,CW,T

C

[39, 95]

rpsD 30S ribosomal protein S4

139

011_1260

046_1365

SAOV_1706

10,02

22999

428,22

40,50

8

1,96

T

C

[95]

rplB 50S ribosomal protein L2

91

011_0528

046_1188

SAOV_2284c

10,77

30136

239,93

25,27

5

0,69

CW

C

[95]

tsf elongation factor Ts

100, 64

011_0909

046_0755

SAOV_1259

5,05

32474

436,02

31,06

7

0,97

CW,S

C

[4, 86, 93, 96]

tuf elongation factor Tu

90, 88, 96, 10, 122, 137, 149, 81, 82, 83, 95

011_0402

046_1768

SAOV_0583

4,74

43077

2665,48

84,26

38

52,14

S,CW,T

C

[3, 52, 82, 85, 86, 90, 93, 96]

yfiA ribosomal subunit interface protein

106

011_2483

046_1255

SAOV_0789

5,25

21511

376,75

35,33

6

1,76

CW

C

 

aspS aspartyl-tRNA synthetase

63

011_2454

046_2303

SAOV_1627

4,99

66584

233,90

7,65

4

0,21

S

C

 

alaS alanyl-tRNA synthetase

82

011_2466

046_2291

SAOV_1616

5,00

98604

442,31

11,74

8

0,30

O

C

[97]

Transcription

             

nusA transcription elongation factor NusA

62

011_0919

046_0765

SAOV_1268

4,60

43701

247,74

11,00

4

0,34

S

C

 

DNA replication, recombination and repair

             

dnaN DNA polymerase III subunit beta

90

011_1166

046_1471

SAOV_0002

4,66

41888

706,28

45,62

11

1,30

CW

C

 

nuc staphylococcal thermonuclease precursor

151, 108, 5, 153, 206, 207, 217

011_2070

046_2528

SAOV_0832

9,20

25089

967,00

50,00

20

14,46

S,CW

PSE

[98]

ruvA Holliday junction DNA helicase

66

011_2442

046_2315

SAOV_1639

5,77

22249

137,72

17,00

3

0,52

S

C

 

Posttranslational modification, protein turnover, chaperones

             

ahpC alkyl hydroperoxide reductase subunit C

67, 82, 83, 99, 95

011_0085

046_0968

SAOV_0404c

4,88

20963

667,36

56,08

11

4,95

S,CW

C

[93]

AhpF ALKYL HYDROPEROXIDE REDUCTASE SUBUNIT F (O11)

97, 86

011_0086

046_0969

SAOV_0403c

4,68

54655

2242,14

67,06

34

9,91

CW

C

 

dnaK chaperone protein

96, 173, 137

011_2230

046_2216

SAOV_1580

4,63

46021

2907,83

80,29

43

24,66

S,CW,T

C

[90, 96, 99]

peptidyl-prolyl cis-isomerase

212, 1, 68, 91

011_2089

046_2477

SAOV_1837

9,01

35602

574,32

31,56

11

1,66

S,CW

PSE

 

tig trigger factor

105

011_1304

046_0602

SAOV_1664

4,34

48565

1061,87

61,43

17

2,25

CW

C

[94]

TrxB THIOREDOXIN REDUCTASE (O11)

64

011_2580

046_2228

SAOV_0801

5,21

33595

681,95

33,76

11

1,81

S

C

[93]

Cellular processes

             

Cell envelope biogenesis, outer membrane

             

IsaA IMMUNODOMINANT ANTIGEN A (O46)

185, 8, 161, 186, 9, 164, 189

011_0168

046_0166

SAOV_2614c

5,91

24219

1516,56

59,23

22

40,99

S

S

[3, 75]

isdA iron-regulated cell wall-anchored protein

31, 27, 73, 74, 75, 83, 118, 79, 81, 82, 95

011_1476

046_1296

SAOV_1125c

8,69

70445

2288,12

57,87

38

6,04

S,CW

PSE

[100–102]

isdB cell surface transferrin-binding protein

212, 211, 210, 208, 193, 156, 138, 110, 70, 86, 131, 156, 193

011_1477

046_1295

SAOV_1126c

9,54

39197

886,30

45,48

15

4,45

S,CW,T

PSE

[54, 100, 103]

IRON-REGULATED SURFACE DETERMINANT PROTEIN H (O11)

55, 31

011_1248

046_1353

SAOV_1717

5,05

100650

2209,72

42,11

37

2,58

S

PSE

[75]

IsdD iron-regulated protein

15, 16

011_1480

046_1292

SAOV_1128

8,51

41357

278,05

15,36

5

0,47

S

PSE

 

Cell motility and secretion

             

N-acetylmuramoyl-L-alanine amidase

52, 51, 187

011_1090

046_1546

SAOV_2693

5,87

69226

3097,93

71,57

43

16,52

S

S

[57, 75, 76]

Inorganic ion transport and metabolism

             

nasE assimilatory nitrite reductase

10

011_1932

046_0245

SAOV_2445c

4,95

11430

126,05

23,08

2

0,70

S

C

 

mntC Manganese/iron transport system substrate-binding protein

94

011_2274

046_0062

SAOV_0666c

8,68

34719

1183,08

51,46

20

10,68

S,CW

PSE

 

sirA iron-regulated lipoprotein

135, 64

011_1345

046_1405

SAOV_0062

9,20

36735

609,79

35,45

11

1,58

S,T

PSE

[104]

fhuD2 ferrichrome-binding protein

91

011_0566

046_1150

SAOV_2323c

9,16

33990

406,75

30,13

8

1,10

CW

PSE

[105]

ferrichrome ABC transporter lipoprotein

91

011_1857

046_1674

SAOV_2224c

9,44

36751

600,45

38,41

11

1,57

CW

PSE

 

Signal transduction mechanisms

             

SA1540 GAF domain-containing protein

10

011_1261

046_1366

SAOV_1705

5,09

17042

139,34

22,73

3

0,72

S

C

 

Universal stress response protein

7, 123

011_1269

046_1374

SAOV_1697

5,60

18463

973,68

78,31

12

19,34

S,CW

C

[106]

Toxins and haemolysins

             

beta-hemolysin

15, 19, 205

011_1750

046_2394

SAOV_2040

8,75

37386

1308,79

61,03

20

4,92

S

S

[57, 107]

hla alpha-hemolysin precursor

13, 1, 15, 19, 21, 68, 72, 107, 146, 153

011_1514

046_1259

SAOV_1161c

8,87

36329

2238,72

76,71

34

44,90

S,CW,T

S

[93, 107]

hlgC gamma-hemolysin component C

68, 1

011_1956

046_0268

SAOV_2469

9,29

35562

765,07

31,43

12

1,90

S,CW

S

[57]

Virulence/defence mechanisms

             

Sbi IGG-BINDING PROTEIN SBI (O11)

216, 217, 212

011_1954

046_0266

SAOV_2466

9,38

49998

984,16

41,74

18

2,80

S

S

[76]

SspB CYSTEINE PROTEASE PRECURSOR (O11)

58, 57, 64, 182, 215

011_2154

046_0327

SAOV_0993c

5,45

42714

2027,86

65,52

30

11,52

S

S

[4]

SplF serine proteinase

4

011_0672

046_2496

SAOV_1795

9,36

25638

255,88

23,01

5

0,84

S

S

[108]

lukD leukotoxin D subunit

1

011_0685

046_2484

SAOV_1812

9,14

36936

365,37

22,02

7

0,98

S

S

[109]

lukE leukotoxin E subunit

1, 68

011_0686

046_2483

SAOV_1813c

9,38

34126

451,11

17,65

7

1,77

S,CW

S

[109]

leukocidin chain lukM precursor

68, 1, 120, 145, 177, 194, 201, 202, 94

011_1215

046_2777

SAOV_1909

9,41

35054

2504,69

71,43

35

29,80

S,CW,T

S

[58, 110]

leukocidin F subunit

16, 70

011_1752

046_1972

SAOV_2041

8,29

38639

1154,65

45,27

19

11,64

S,CW

S

[58, 110]

leukocidin S subunit

199, 5, 70, 108, 109, 197, 200, 211

011_1753

046_1973

SAOV_2042

9,38

40379

1399,91

55,56

23

6,70

S,CW

S

[58, 110]

Panton-Valentine leukocidin LukF-PV chain precursor

1, 68, 70, 15, 31, 119, 195, 203, 204, 10, 91

011_1216

046_2776

SAOV_1908

9,16

36496

965,38

50,31

15

3,37

S,CW

S

[58, 110]

plc 1-phosphatidylinositol phosphodiesterase

11, 12, 19

011_1424

046_1419

SAOV_0049

7,12

37030

2759,20

71,95

44

84,00

S

S

[4]

Aur ZINC METALLOPROTEINASE AUREOLYSIN (O11)

220, 63

011_1083

046_1553

SAOV_2686c

4,98

54947

921,93

47,39

17

1,68

S

C

[76, 111]

Opp1A OLIGOPEPTIDE TRANSPORTER SUBSTRATE BINDING PROTEIN (O11)

86

011_2424

046_2102

SAOV_2517c

8,33

59224

1398,01

47,53

24

3,28

CW

PSE

 

SspA GLUTAMYL ENDOPEPTIDASE SERINE PROTEASE (O11)

56, 184, 214

011_2155

046_0325

SAOV_0994c

4,68

32250

1575,55

69,26

24

24,18

S

C

[57]

SECRETED VON WILLEBRAND FACTOR-BINDING PROTEIN (O11)

22

011_2679

046_0987

SAOV_2051c

8,39

57935

1122,89

36,67

17

1,70

S

S

 

epidermal cell differentiation inhibitor B

208, 209, 193, 156, 4

011_0489

046_2741

 

9,51

27969

1385,18

69,32

22

22,31

S

S

 

Miscellaneous

             

adhA alcohol dehydrogenase

100

011_1554

046_0086

SAOV_0640

5,34

36025

1167,82

66,37

16

4,30

CW

C

[87, 93]

exported secretory antigen precursor

67

011_0584

046_1132

SAOV_2343

5,77

17388

397,17

33,13

5

1,43

S

S

 

lip triacylglycerol lipase precursor

31, 27

011_1119

046_1518

SAOV_2721c

8,13

76637

1723,17

50,95

29

2,36

S

S

[93]

mqo malate:quinone oxidoreductase

128

011_0130

046_0203

SAOV_2651c

6,12

55964

1047,99

46,79

17

2,50

T

C

 

putative staphylococcal enterotoxin

151

011_0097

046_0981

SAOV_0394

9,54

23311

173,20

18,72

4

0,71

S

S

 

Unknown

             

HYPOTHETICAL PROTEIN (O11)

220

011_0736

046_1969

SAOV_0454

4,89

56229

823,11

39,40

14

1,21

S

S

 

hypothetical protein (Similar to truncated map-w protein (91%))

88, 10, 62

011_1749

046_2393

 

9,91

53686

899,64

34,52

18

2,09

S,CW

S

[112]

hypothetical protein (Similar to beta-lactamase (84%))

10

011_0679

046_2490

 

6,84

20800

726,52

47,15

10

7,09

S

S

 

HYPOTHETICAL PROTEIN (O46)

157, 160, 175, 189, 193

011_0490

046_2740

 

8,88

30854

2343,27

74,29

41

65,07

S

S

 

hypothetical protein (Similar to probable glutamyl-endopeptidase (76%))

66

011_0488

046_2742

 

5,44

20552

741,93

47,62

12

5,11

S

C

 

SA0570 HYPOTHETICAL PROTEIN (O46)

219,154, 127

011_2290

046_0078

SAOV_0649

9,17

18554

815,59

69,05

13

15,93

S,CW

S

 

SA0663 hypothetical protein

10, 164, 94

011_2561

046_2636

SAOV_0742c

9,15

16047

457,11

35,62

8

3,63

S

PSE

 

SA0914 hypothetical protein

6

011_2000

046_0311

SAOV_1008c

6,55

11338

522,48

57,14

8

13,10

S

S

 

pathogenicity island protein

55

011_2741

046_0985

SAOV_0429

9,55

12071

204,61

19,64

3

1,12

S

S

 

SA1607 hypothetical protein

91

011_1867

046_2456

SAOV_1770

6,04

34973

232,41

16,56

4

0,43

CW

S

 

SA1402 hypothetical protein

91

011_2223

046_2209

SAOV_1573

5,60

35160

448,93

28,27

6

0,72

CW

S

 
  1. Gene products of the accessory seroproteome are indicated in bold capital letters, (O11) indicates O11-specific proteins, (O46) indicates O46-specific proteins.
  2. All the other proteins belong to the core seroproteome
  3. 1: Proteins are classified in Gene Ontology functional classes. Names are given according to annotation of available S. aureus sequence genomes.
  4. 2: Spot number (see figures)
  5. 3: Coding sequence numbers corresponding to the identified proteins in S. aureus O11, S. aureus O46, and ED133, respectively.
  6. 4: Theoretical isoelectric point as determined from the predicted protein sequence
  7. 5: Theoretical Mass as determined from the predicted protein sequence
  8. 6: Mascot standard score
  9. 7: % of the protein sequence covered by the peptides identified
  10. 8: number of peptides identified
  11. 9: exponentially modified protein abundance index
  12. 10: Subproteome compartment where the spot was identified. CW, cell wall; S, supernatant; T, total fraction
  13. 11: predicted protein localization. C, cytoplasmic; S, secreted; PSE, predicted surface exposed
  14. 12: reported as immunogen elsewhere
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Veterinary Research

ISSN: 1297-9716