Figure 6

Distribution of ovine NKp46⁺ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46⁺ cells: green; B-cells (IgM⁺) red; CD4⁺ T-cells blue in panel A and B; Epithelial cells (E-cadherin⁺) blue in parts C-H. White arrows: individual NKp46⁺ cells. Red arrows: putative intra-epithelial NKp46⁺ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.