Table 4 In situ probes used for visualisation of Francisella spp. in aquatic animals.
From: Francisella infections in farmed and wild aquatic organisms
Fish type | Bacterial species | Probe type¤ | Primers or probes (5' - 3') | Reference |
|---|---|---|---|---|
Cichlids | Francisella spp. | PCR product (286bp) | FLB16S180f gcg-gat-taa-agg-tgg-cct-ttg-c | Hsieh et al. [42] |
| Â | Â | Â | FLB16S465r cct-gca-agc-tat-taa-ctc-aca-gg | Â |
Tilapia | Francisella sp.* | PCR product (1113bp) | F5 cct-ttt-tga-gtt-tcg-ctc-c | Hsieh et al. [2]# |
| Â | Â | Â | F11 tac-cag-ttg-gaa-acg-act-gt | Â |
Three-lined grunt | Francisella noatunensis subsp. orientalis | Antisense oligo- | Isaki-DIG-80r ctc-gtc-agc-atc-cga-aga-cct-gtt-a | Kamaishi et al. [3] |
| Â | Â | Â | Isaki-DIG-200r ggc-agc-gca-aag-gcc-acc-ttt-aat-ccg-cag-ata-t | Â |
Abalone | Francisella sp. | Antisense oligo- | Megai-110r ccg-cca-ctc-gtc-agc-aag-aag-caa-gct-tct-cct-gtt-acc-gtt-cga-ctt-gc | Kamaishi et al. [8] |
| Â | Â | Â | Megai-230r cta-acg-cag-gct-cat-cca-tct-gcg-gca-gca-caa-agg-cca-cct-tta-atc-ctc-aga-tag-tat | Â |
| Â | Â | Â | Megai-870r gag-tac-tta-acg-cgt-tag-cta-cgc-cac-tag-atc-ctt-tac-acc-gaa-tcc-aac-agc-tag-tac | Â |