Table 1 The ELISA kit, anti-cytokine antibodies (ab) and cytokine protein standards used in this study.
Cytokine | ELISA kit/antibody/protein standard | Source | Final concentration and buffer |
|---|---|---|---|
IL-8 | Human IL-8 ELISA kit | DY208 R&D Systems, Minneapolis, MN | |
IL-8 | Recombinant bovine IL-8 | RPOIL8I Thermo Scientific, Waltham, MA | |
IL-1β | Mouse anti-sheep IL-1β (capturing ab) | MCA1658 AbD Serotech, Oxford, UK | 4 μg/mL in PBS |
IL-1β | Recombinant bovine IL-1β | PBP008 AbD Serotech | |
IL-1β | Biotin-conjugated mouse anti-bovine IL-1β (detection ab) | AHP851B AbD Serotech | 2 μg/mL in 1% BSA-PBS |
TNF-α | Mouse anti-bovine TNF-α (capturing ab) | MCA2334 AbD Serotech | 1 μg/mL in PBS |
TNF-α | Recombinant bovine TNF-α | PBP005 AbD Serotech | |
TNF-α | Biotin conjugated mouse anti-bovine TNF-α (detection ab) | MCA2335B AbD Serotech | 2.5 μg/mL in 1% BSA-PBS |