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Table 2 List of genes and sequences of the primers used for real-time PCR1.

From: Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs

mRNA target

Primers (5' → 3')2

Product size (bp)

Final concentration (nM)3

IL-4

F:GGTCTGCTTACTGGCATGTACC

117

150

 

R:CTCCATGCACGAGTTCTTTCTC

 

150

IL-6

F:GGAAATGTCGAGGCTGTGCAGATT

87

300

 

R:GGTGGTGGCTTTGTCTGGATTCTT

 

300

IL-8

F:AGAACTGAGAAGCAACAACAACAG

132

300

 

R:CACAGGAATGAGGCATAGATGTAG

 

300

IL-10

F:GATATCAAGGAGCACGTGAACTC

137

300

 

R:GAGCTTGCTAAAGGCACTCTTC

 

300

IL-12p35

F:TGCAGGCTCTGAATTTCAAC

111

150

 

R:CACGAATTCTGAAGGCATGA

 

150

IL-12p40

F:CTTCATCAGGGACATCATCAAAC

196

300

 

R:GGTCCGTGAAGAGTTTATCTTTCT

 

150

IFN-γ

F:AGGTTCCTAAATGGTAGCTCTGGG

101

300

 

R:AGTTCACTGATGGCTTTGCGCT

 

300

TNF-α

F:CACTGACCACCACCAAGAATTGGA

94

300

 

R:CATTCCAGATGTCCCAGGTTGCAT

 

300

pBD-2

F:CCGACCACTACATATGTGCCAAGA

93

300

 

R:TGCCACTGTAACAGGTCCCTTCAA

 

300

COX-2

F: AAGCGAGGACCAGCTTTCACCAAA

93

300

 

R:GCGCAGTTTATGCTGTCTCTCCAA

 

300

ACTB

F:CTCTTCCAGCCCTCCTTCCT

104

300

 

R:GCGTAGAGGTCCTTCCTGATGT

 

300

  1. 1IL = interleukin; IFN = interferon; TNF = tumor necrosis factor; pBD-2 = porcine β-defensin-2; COX-2 = cyclooxygenase-2; ACTB = β-actin.
  2. 2F and R indicate forward and reverse primers, respectively.
  3. 3Final concentration of forward (F) and reverse (R) primers.