Figure 2

Phylogenetic relationships of deer Babesia species (present study in bold) according to the sequence amplified by PCR protocol I (bp loci 527-1005 in reference sequence AY046576*). The tree was constructed by sequence alignment of the 18S rRNA gene fragment and neighbour-joining analysis. Tree reliability was assessed by the bootstrap method with 1 000 pseudoreplicates (MEGA version 3.1, [14]). (*all amplicons exclude the primer sequences (as they are not part of the original DNA template) and approx. 40 bp at the 5' region for which there was insufficient over-lapping sequencing data).