Figure 3
Immunohistochemical detection of Neospora caninum developmental stages in fat-tailed dunnart. Positive IHC staining with anti-Neospora antibodies: staining of elongated tissues cysts of N. caninum in cardiac (a) and skeletal muscle (b); pancreas containing a large number of free zoites (c) and multiple round cysts within a single acinar cell (d, e); lymph node (f), brain (g) and lung (h) with scattered developmental stages including free zoites; lung tissue with a large round cyst filled with zoites within a large mononuclear cell likely to be a pulmonary macrophage (i); and a cross-section of the urinary bladder wall with N. caninum stages apparent in all layers of the detrusor muscle (j). Tissues from animals inoculated 105 N. caninum tachyzoites (B11: A, H, I; B21: B-G, J). Bars: A, B, F, G, H = 20 μm; C = 50 μm; D+E, I = 10 μm, and J = 100 μm.