Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta

Knight, Pamela A; Griffith, Susan E; Pemberton, Alan D; Pate, Judith M; Guarneri, Lauren; Anderson, Katherine; Talbot, Richard T; Smith, Sarah; Waddington, David; Fell, Mark; Archibald, Alan L; Burgess, Stewart TG; Smith, David W; Miller, Hugh RP; Morrison, Ivan W

doi:10.1186/1297-9716-42-78

Veterinary Research

Table 5 The 20 most down-regulated transcripts detected in Expts 1 and 2 (FDR ≤ 0.05)

From: Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta

Sequence.ID	TIGR_TC	TC.description	Gene symbol	Fold Change
EXPT 1 Comparison = d5Nv_d0Nv
KN511_9487m11.p1kM13F	TC336019	UP\|MMP13_BOVIN (O77656) Collagenase 3 precursor (Matrix metalloproteinase-13) (MMP-13), complete	MMP13	-2.68
KN511_9481c03.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-2.37
KN511_9476d13.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-2.33
KN511_9485a08.p1kM13F	TC302650	milk lysozyme [Bos taurus]	LYZ1	-2.30
KN511_9485g08.p1kM13F	TC346615	similar to UP\|O97916_BOVIN (O97916) Reverse transcriptase-like, partial (32%)	---	-2.23
KN511_9257d12.p1kaM13F	TC322737	similar to UP\|Q3VD89_9SPHN (Q3VD89) ABC-2, partial (5%)	---	-2.16
KN511_9257a18.p1kaM13F	TC308928	similar to GB\|AAK18773.1\|13324523\|F272846S31 Fanconi anemia complementation group D2 protein, isoform 2 {Homo sapiens} (exp = -1; wgp = 0; cg = 0), partial (18%)	LOC515845	-2.14
KN511_9262d01.p1kaM13F	---	---	---	-2.12
KN511_9261n23.p1kaM13F	TC362037	homologue to UP\|IFRD1_PIG (Q5S1U6) Interferon-related developmental regulator 1, complete	IFRD1	-2.12
KN511_9476d14.p1kaM13F	TC349311	similar to UP\|NEK4_HUMAN (P51957) Serine/threonine-protein kinase Nek4 (NimA-related protein kinase 4) (Serine/threonine-protein kinase 2) (Serine/threonine-protein kinase NRK2), partial (33%)	MGC159441	-2.08
KN511_9261o08.p1kaM13F	TC305060	homologue to UP\|Q96BA7_HUMAN (Q96BA7) HNRPU protein, partial (93%)	MGC142835	-2.07
KN511_9258d23.p1kaM13F	---	---	---	-2.07
KN511_9487p21.q1kM13R	TC342358	homologue to UP\|DC1L2_HUMAN (O43237) Cytoplasmic dynein 1 light intermediate chain 2 (Dynein light intermediate chain 2, cytosolic) (LIC53/55) (LIC-2), complete	LOC519789	-2.05
KN511_9262k18.p1kaM13F	TC375209	similar to UP\|ZSWM3_HUMAN (Q96MP5) Zinc finger SWIM domain-containing protein 3, partial (27%)	LOC512244	-2.05
KN511_9485d23.q1kM13R	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-2.04
KN511_9254g13.p1kaM13F	TC319944	---	---	-2.02
KN511_9487h17.p1kM13F	TC327406	UP\|Q5DTN6_MOUSE (Q5DTN6) MKIAA4095 protein (Fragment), partial (8%)	LOC614823	-1.96
CO202908	TC360504	UP\|Q3MHM4_BOVIN (Q3MHM4) Heat shock 70 kDa protein 8, complete	HSPA8	-1.87
KN511_9482g13.p1kM13F	TC331274	homologue to UP\|TENA_PIG (Q29116) Tenascin precursor (TN) (Hexabrachion) (Cytotactin) (Neuronectin) (GMEM) (JI) (Miotendinous antigen) (Glioma-associated-extracellular matrix antigen) (GP 150-225) (Tenascin-C) (TN-C) (P230), partial (10%)	MGC140517	-1.87
KN511_9482f05.p1kM13F	TC377013	---	---	-1.86
EXPT 1 Comparison = d5Im_d5Nv
KN511_9256g17.p1kaM13F	TC314535	homologue to UP\|Q94513_DROME (Q94513) Boundary element associated factor (LD44361p) (CG10159-PB, isoform B), partial (5%)	---	-6.51
KN511_9256g17.p1kaM13F	TC314535	homologue to UP\|Q94513_DROME (Q94513) Boundary element associated factor (LD44361p) (CG10159-PB, isoform B), partial (5%)	---	-5.97
KN511_9254l23.p1kaM13F	TC354719	similar to UP\|TFF2_HUMAN (Q03403) Trefoil factor 2 precursor (Spasmolytic polypeptide) (SP) (Spasmolysin), complete	MGC139191	-5.54
KN511_9257l15.p1kaM13F	TC354719	similar to UP\|TFF2_HUMAN (Q03403) Trefoil factor 2 precursor (Spasmolytic polypeptide) (SP) (Spasmolysin), complete	MGC139191	-5.42
KN511_9260b10.p1kaM13F	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.92
KN511_9260m19.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.79
KN511_9483j03.p1kM13F	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.52
KN511_9265j02.p1kaM13F	CB220717	---	---	-3.45
KN511_9264e04.p1kaM13F	TC314849	UP\|Q6SJV6_BOVIN (Q6SJV6) Foveolin precursor, complete	FOV	-3.33
KN511_9262k17.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.25
KN511_9476c18.p1kaM13F	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.25
KN511_9476d13.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.17
KN511_9488a15.p1kM13F	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.16
KN511_9259k01.p1kaM13F	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.14
KN511_9265m20.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.10
KN511_9263j17.p1kaM13F	TC323852	similar to UP\|Q86XP6_HUMAN (Q86XP6) GDDR (Blottin precursor) (TFIZ1 protein precursor), complete	LOC512001	-3.10
KN511_9487e19.p1kM13F	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.08
KN511_9485d23.q1kM13R	TC365645	UP\|LYSC2_BOVIN (Q06283) Lysozyme C-2 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-3.05
KN511_9486h17.p1kM13F	TC314849	UP\|Q6SJV6_BOVIN (Q6SJV6) Foveolin precursor, complete	FOV	-3.04
KN511_9265h22.p1kaM13F	TC304583	UP\|LYSC1_BOVIN (Q06285) Lysozyme C-1 precursor (1,4-beta-N-acetylmuramidase C), complete	LYZ2	-2.99
EXPT 2 Comparison = Day 0 Im vs Day 0 Nv
KN511_9484p13.p1kM13F	TC376600	similar to UP\|CENPA_MOUSE (O35216) Centromere protein A (CENP-A) (Centromere autoantigen A), partial (15%)	CENP-A	-1.75
KN511_9479i16.p1kaM13F	---	---	---	-1.75
KN511_9253l11.p1kaM13F	TC303509	homologue to UP\|RIR2_HUMAN (P31350) Ribonucleoside-diphosphate reductase M2 subunit (Ribonucleotide reductase small subunit) (Ribonucleotide reductase small chain), partial (88%)	RRM2	-1.73
KN511_9488d17.p1kM13F	TC314207	similar to UP\|TOP2A_PIG (O46374) DNA topoisomerase 2-alpha (DNA topoisomerase II, alpha isozyme), partial (17%)	TOP2A	-1.71
KN511_9265l09.p1kaM13F	TC340393	UP\|CCNA2_BOVIN (P30274) Cyclin-A2 (Cyclin-A) (Fragment), complete	CCNA2	-1.70
KN511_9488f20.p1kM13F	TC355225	homologue to UP\|BIRC5_CANFA (Q8I009) Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor survivin), complete	BIRC5	-1.66
KN511_9487m04.p1kM13F	TC355225	homologue to UP\|BIRC5_CANFA (Q8I009) Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor survivin), complete	BIRC5	-1.65
KN511_9253i15.p1kaM13F	TC340307	UP\|CP51A_BOVIN (Q4PJW3) Cytochrome P450 51A1 (CYPLI) (P450LI) (Sterol 14-alpha demethylase) (Lanosterol 14-alpha demethylase) (LDM) (P450-14DM) (P45014DM), complete	CYP51	-1.63
KN511_9265a16.p1kaM13F	TC303827	---	KIF11	-1.58
KN511_9264b09.p1kaM13F	---	---	---	-1.56
KN511_9256j19.p1kaM13F	TC355225	homologue to UP\|BIRC5_CANFA (Q8I009) Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor survivin), complete	BIRC5	-1.53
KN511_9261d22.p1kaM13F	---	---	---	-1.50
KN511_9481l06.p1kaM13F	TC303509	homologue to UP\|RIR2_HUMAN (P31350) Ribonucleoside-diphosphate reductase M2 subunit (Ribonucleotide reductase small subunit) (Ribonucleotide reductase small chain), partial (88%)	LOC508167	-1.49
KN511_9476j14.p1kaM13F	TC333525	homologue to GB\|AAH08056.2\|39644830\|BC008056 PLEK2 protein {Homo sapiens} (exp = -1; wgp = 0; cg = 0), partial (21%)	MGC142714	-1.48
KN511_9482e10.p1kM13F	TC330132	similar to UP\|CV106_HUMAN (Q6P0N0) Protein C14orf106 (P243), partial (33%)	LOC507661	-1.47
KN511_9257p16.p1kaM13F	TC340307	UP\|CP51A_BOVIN (Q4PJW3) Cytochrome P450 51A1 (CYPLI) (P450LI) (Sterol 14-alpha demethylase) (Lanosterol 14-alpha demethylase) (LDM) (P450-14DM) (P45014DM), complete	CYP51	-1.46
KN511_9256f06.p1kaM13F	TC332145	similar to UP\|Q9BDH4_PIG (Q9BDH4) Amphiregulin long form, complete	MGC152310	-1.45
CN824006	TC306385	homologue to UP\|Q4S6A0_TETNG (Q4S6A0) Chromosome 9 SCAF14729, whole genome shotgun sequence, partial (59%)	LOC404053	-1.42
KN511_9257i02.p1kaM13F	TC327524	homologue to UP\|Q9BW51_HUMAN (Q9BW51) TTK protein kinase, partial (44%)	LOC524925	-1.41
KN511_9261e22.p1kaM13F	TC301804	homologue to UP\|IMA2_HUMAN (P52292) Importin alpha-2 subunit (Karyopherin alpha-2 subunit) (SRP1-alpha) (RAG cohort protein 1), complete	KPNA2	-1.41
EXPT 2 Comparison = Day 2 Im vs Day 0 Im
KN511_9479k18.p1kaM13F	CX950602	---	---	-3.53
KN511_9488b02.p1kM13F	DY126632	---	---	-3.24
KN511_9257l15.p1kaM13F	TC354719	similar to UP\|TFF2_HUMAN (Q03403) Trefoil factor 2 precursor (Spasmolytic polypeptide) (SP) (Spasmolysin), complete	TFF2	-3.14
KN511_9254l23.p1kaM13F	TC354719	similar to UP\|TFF2_HUMAN (Q03403) Trefoil factor 2 precursor (Spasmolytic polypeptide) (SP) (Spasmolysin), complete	TFF2	-3.03
KN511_9264e04.p1kaM13F	TC314849	UP\|Q6SJV6_BOVIN (Q6SJV6) Foveolin precursor, complete	FOV	-2.81
KN511_9263j17.p1kaM13F	TC323852	similar to UP\|Q86XP6_HUMAN (Q86XP6) GDDR (Blottin precursor) (TFIZ1 protein precursor), complete	GKN2	-2.45
KN511_9486h17.p1kM13F	TC314849	UP\|Q6SJV6_BOVIN (Q6SJV6) Foveolin precursor, complete	FOV	-2.09
KN511_9261k22.p1kaM13F	TC327109	similar to UP\|O97916_BOVIN (O97916) Reverse transcriptase-like, partial (21%)	---	-1.93
KN511_9486b13.p1kM13F	TC341767	---	---	-1.90
KN511_9484h13.p1kM13F	TC350450	similar to UP\|UDB5_MOUSE (P17717) UDP-glucuronosyltransferase 2B5 precursor (UDPGT) (M-1), partial (49%)	UGT2B17	-1.81
KN511_9263c22.p1kaM13F	---	---	---	-1.80
KN511_9264a19.p1kaM13F	---	---	---	-1.77
KN511_9484l14.p1kM13F	TC362885	similar to UP\|MALAT_HUMAN (Q9UHZ2) Metastasis-associated lung adenocarcinoma transcript 1, partial (72%)	MALAT1	-1.75
KN511_9480l06.p1kaM13F	TC362885	similar to UP\|MALAT_HUMAN (Q9UHZ2) Metastasis-associated lung adenocarcinoma transcript 1, partial (72%)	MALAT1	-1.74
KN511_9481o23.p1kaM13F	TC350450	similar to UP\|UDB5_MOUSE (P17717) UDP-glucuronosyltransferase 2B5 precursor (UDPGT) (M-1), partial (49%)	UGT2B17	-1.73
CN824758	---	---	---	-1.72
KN511_9488a17.p1kM13F	TC318925	---	---	-1.71
KN511_9480o03.p1kaM13F	---	---	---	-1.70
KN511_9263o20.p1kaM13F	TC380127	similar to UP\|UDB5_MOUSE (P17717) UDP-glucuronosyltransferase 2B5 precursor (UDPGT) (M-1), partial (42%)	UGT2B17	-1.70
KN511_9484i14.p1kM13F	TC362885	similar to UP\|MALAT_HUMAN (Q9UHZ2) Metastasis-associated lung adenocarcinoma transcript 1, partial (72%)	MALAT1	-1.67

The experiment and the samples being compared are given as subheadings at the top of each section. Nv = "naïve" yearlings, worm naïve prior to challenge; Im = "immune" yearlings, previously infected by trickle infection prior to challenge, d = days post-challenge. Details of the experimental trials and hybridisation design are given in Tables 1 and 2.

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ISSN: 1297-9716

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