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Table 2 Transcription stability of reference genes illustrated by the Ct ranges obtained from each tissue throughout 6 weeks post SPDV challenge (n = 20).

From: Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection

Gene

Ct range (fold change)

 

Head kidney

Heart

Intestine

Spleen

Gills

EF1αB

19.6-22.4 (6.9X)

19.5-22.4 (7.4X)

18.4-21.2 (6.9X)

19.3-24.2 (29.8X)

19.3-21.6 (4.9X)

18S rRNA

12.9-15.3 (5.2X)

12.5-14.9 (5.2X)

12.7-14.1 (2.6X)

12.4-17.3 (29.8X)

12.5-15.6 (8.5X)

β-actin

19.4-22.4 (8.0X)

18.8-22.8 (16.0X)

18.0-21.4 (10.5X)

18.5-23.0 (22.6X)

19.2-21.3 (4.2X)

RPS20

25.2-28.4 (9.1X)

23.3-25.5 (4.5X)

23.2-26.6 (10.5X)

21.4-27.7 (78.7X)

25.7-28.5 (6.9X)

  1. Level of target gene transcription regulation (fold change) that can be significantly identified using the respective reference genes is shown in brackets.