Table 2 Primers used for PCR amplification and sequencing of domains II and V of 23S rRNAs and ribosomal proteins of L4 and L22 in M. gallisepticum.
Primer designation | Source of primer | Sequence (5'-3') | Nucleotide positiona | Amplicon size (bp) |
|---|---|---|---|---|
Domain V of MGAr01 | ||||
MG-23S-1F1 MG-0754-R | MGAr01 MGA_0754 | CACAGCTCTATGCTAAATCGC GATAATTGGTGGAGTTGG | 82312-82332 and 324019-324039 83691-83708 | 1396 |
Nested PCR for domain V of MGAr01 | ||||
MG-23S-1F1 MG-23S-1R2 | MGAr01 MGAr01 | CACAGCTCTATGCTAAATCGC GGTCCTCTCGTACTAAG | 82312-82332 and 324019-324039 83175-83191 and 324882-3244898 | 879 |
Domain II of MGAr01 | ||||
MG-mdh-F MG-rRNAII-R3 | MGA_0746 MGAr01 | GCAAGCACGGATGGAAGT CCACTGTCTGACTGCAAG | 79928-79945 81483-81500 and 323190-323207 | 1572 |
Nested PCR fordomain II of MGAr01 | ||||
MG-rRNAII-F4 MG-rRNAII-R3 | MGAr01 MGAr01 | GGTTTAATACCTAGCAGGAT CCACTGTCTGACTGCAAG | 80893-80912 and 322600-322619 81483-81500 and 323190-323207 | 607 |
Domain V of MGAr04 | ||||
MG-23S-1F1 MG-1046-R | MGAr04 MGA_1046 | CACAGCTCTATGCTAAATCGC GCTAATTGCCTCCTGGTAAC | 324019-324039 and 82312-82332 325669-325688 | 1669 |
Nested PCR for domain V of MGAr04 | ||||
MG-23S-1F1 MG-23S-1R2 | MGAr04 MGAr04 | CACAGCTCTATGCTAAATCGC GGTCCTCTCGTACTAAG | 324019-324039 and 82312-82332 324882-324898 and 83175-83191 | 879 |
Domain II of MGAr04 | ||||
MG-16S-F MG-rRNAII-R3 | MGAr03 MGAr04 | GGAATCACTAGTAATCGC CCACTGTCTGACTGCAAG | 321355-321317 323190-323207 and 81483-81500 | 1852 |
Nested PCR for domain II of MGAr04 | ||||
MG-rRNAII-F4 MG-rRNAII-R3 | MGAr04 MGAr04 | GGTTTAATACCTAGCAGGAT CCACTGTCTGACTGCAAG | 322600-322619 and 80893-80912 323190-323207 and 81483-81500 | 607 |
rplD (L4) | ||||
MG-L4-F MG-L4-R | MGA_0710 MGA_0710 | CGATTTATCTGGAAAAGTTCAAG GTTCAACCTTTCAACTCAGTTAT | 67500-67522 68084-68106 | 606 |
rplV (L22) | ||||
MG-L22-F MG-L22-R | MGA_0716 MGA_0716 | ATGATCGCAATTGCAAGACAA CTCCGCTAACTGATTGTTTTC | 69631-69651 70034-70054 | 423 |