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Figure 1 | Veterinary Research

Figure 1

From: Selection of Neospora caninum antigens stimulating bovine CD4+ve T cell responses through immuno-potency screening and proteomic approaches

Figure 1

Size exclusion fractionation of N. caninum Water Soluble Antigen. (A). Three identical aliquots of NcWSA were divided into fractions comprising molecules of progressively lower mass by size exclusion HPLC. The superimposed absorbance profiles of the fractions generated in three successive runs are shown. Horizontal axis: time, vertical axis: 280 nm absorbance. Fraction collection started 10 min into the run, flow rate was 0.5 mL/min and a new fraction was collected every 2 min. The horizontal dotted lines indicate which portion of the NcWSA antigen corresponded to each collected fraction; bold numbers above the dotted lines represent the fraction number, vertical numbers represent retention time in minutes. (B): Silver stained SDS PAGE gel (4-12% Bis-Tris gradient) of three representative fractions (3, 7 and 11) obtained in three separate runs (.1, .2 and .3) of size-exclusion chromatography. MW: molecular weight markers; 3.1: fraction 3 run 1; 3.2: fraction 3 run 2; 3.3: fraction 3 run 3; the numeration is equivalent for the further two fractions.

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