Selection of Neospora caninum antigens stimulating bovine CD4+ve T cell responses through immuno-potency screening and proteomic approaches

Table 3 Homologues of T. gondii proteins identified in only one fraction

Fraction	Accession	Gene name	Description	MOWSE	Peptides Matched	Example Peptides
3	EEE22451		Putative uncharacterized protein	837	7	K.LEVGETCTIEMLPQNSK.V; K.HKLEVGETCTIEMLPQNSK.V
3	EEE23072		Putative uncharacterized protein	194	4	R.ATVHPGDTVTMQCPGAISSNPADVSK.Y; R.LILDIEKSEEEVVR.T
3	EEE32684		Surface protein rhoptry protein	135	2	K.SQANQGSPLPPPRPNLLR.R; R.GLMSGVGWVK.R
3	EEE29336		Histone H4	110	2	R.ISGLIYEEIR.G; R.DNIQGITKPAIR.R
3	XP_002370897	ROP 2	Rhoptery protein 2	91	3	R.DSGDVILEELFK.R; K.GPSAIVFEATDR.E
3	EEE23774		Ribosomal protein S8	82	2	K.NSIVAIDATPFK.A; K.LDPLLEEQFNTGR.L
4	AAD38419		HSP 60	283	4	K.QVASTTNDIAGDGTTTATLLAR.A; K.TLTHELELVEGLK.F
4	XP_002369317		Proteasome subunit alpha (Type 2)	215	7	R.YNPDIELEDAIHTAILTLK.E; K.EGFEGAMNEHNIEIGVVGEDR.K + Oxidation (M)
4	EEE23454		Proteasome subunit alpha (Type 1)	182	3	K.ELSLDEIQALLDK.M; R.NFESFPGLSPEELELHAMK.A
4	XP_002366589		Proteasome subunit alpha (Type 4)	162	4	K.EDLDVDAALLLAAK.V; K.QEWKEDLDVDAALLLAAK.V
4	EEE19215		Proteasome subunit Beta [(Type 7)	100	2	K.GCAVVLGGVDFK.G; R.VSMAVSVLSQELFK.Y
4	EEE25357		Proteasome subunit alpha (Type 7)	129	2	K.DLVVLAVEK.K; R.LNTATAPSVDYIAK.F
5	EEE30125		Cytosol aminopeptidase putative	142	2	K.LTLFTDDVEAVNR.S R.VVTSFLETLLVELQPDLR.F

Proteins were identified in each fraction by blasting the results of the peptide analysis versus the N. caninum and T. gondii genomic.
Proteins were identified in each fraction by blasting the results of the peptide analysis versus the N. caninum and T. gondii genomic databases. Table 1 lists protein identified from the N. caninum database; Table 2 T. gondii homologues identified in more than one fraction and Table 3 T. gondii homologues present only in one of the three fractions. MOWSE scores (for Molecular Weight Search) indicate the likelihood of having correctly identified a specific protein from the molecular weight of the peptides created by its proteolytic digestion and measured with mass spectrometry. The dot (.) in the peptide sequence denotes trypsin cleavage sites.

ISSN: 1297-9716