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Figure 3 | Veterinary Research

Figure 3

From: Extracellular matrix alterations in experimental Leishmania amazonensis infection in susceptible and resistant mice

Figure 3

Histopathological analysis of footpad skin from mice subcutaneously infected with 104 amastigotes of L. amazonensis. A. BALB/c mice 30 days after infection. Intense inflammatory infiltrate in the reticular dermis (***), with numerous vacuolated macrophages (arrowheads) and polymorphonuclear cells, and slight dissociation of the extracellular matrix fibres (plain arrow) (HE). B. C3H.He mice 30 dpi. Intact papillar dermis and moderate inflammatory infiltrate (***) rich in neutrophils and parasitized macrophages in the reticular dermis (HE). C and D: BALB/c mice 30 days after infection. Picrosirius red in non-polarized (C) and polarized light (D). The image shows type I collagen (red) and a significant production of collagen type III (green) between inflammatory cells present in the reticular dermis. E. BALB/c mice 30 dpi. Window shows elastic (thin arrow) and elaunin fibres (thick arrow) preserved. F. C3H.He mice 60 days after infection. Integrity of elastic (thin arrow) and elaunin (thick arrow) and absence of oxytalan fibres (Weigert). G and H: C3H.He mice 60 days after infection. Picrosirius red in non-polarized (G) and polarized light (H). Attempt to repair infected dermis, with presence of collagens type I (red), III and I neoformed (yellow).

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