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Figure 4 | Veterinary Research

Figure 4

From: Stress significantly increases mortality following a secondary bacterial respiratory infection

Figure 4

CD14 and TLR4 expression in PBMC and LPS-responsiveness following BHV-1 infection. (A) qRT-PCR analysis of CD14 expression levels (ΔCt) in PBMC on day 4 post-BHV-1 infection. Data presented are values for individual animals in weaned and maternal separation (WMS) and pre-adapted (PA) groups (n = 10/group) and horizontal bars represent median values for each group. A lower ΔCt corresponds to greater mRNA abundance. Data was also sorted for calves that died (Dead; n = 12) or survived (Day 0; n = 8) following M. haemolytica infection. (B) qRT-PCR analysis of TLR4 expression levels (ΔCt) in PBMC on day 4 post-BHV-1 infection. Data presented are values for individual animals in the WMS and PA groups (n = 10/group) and horizontal bars represent median values for each group. Data was also sorted for calves that died (Dead; n = 12) or survived (Day 0; n = 8) following M. haemolytica infection. (C) TNF-α secretion by PBMC was measured 24 h after LPS 20 stimulation. PBMC were isolated prior to BHV-1 infection (Day 0) and 2 and 4 days post-BHV-1 infection. Data presented are values for individual animals in the WMS and PA groups (n = 10/group) and horizontal bars represent median values for each group. *p < 0.05; **p < 0.01.

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