Figure 2
Effect of T-2 toxin on the intestinal inflammatory response. Fold change in cytokine gene expression of the porcine ileum of Salmonella Typhimurium positive pigs that received feed contaminated with 15 μg T-2 toxin per kg feed (15 ppb T-2 toxin) or feed contaminated with 83 μg T-2 toxin per kg feed (83 ppb T-2 toxin), relative to Salmonella Typhimurium positive pigs that received blank feed (control group), during 23 days. Five days after inoculation with 2 × 107 CFU of Salmonella Typhimurium, the pigs (n = 5) were euthanized and the cytokine gene expression levels (A: Il-1β, B: Il-6, C: Il-8, D: IL-12, E: IL-18, F: TNFα, G: IFNγ and H: MCP-1) were determined. The data represent the normalized target gene amount relative to the control group which is considered 1. The results are presented as means + standard deviation for a total of 5 pigs per test condition. Superscript (*) refers to a significant difference compared to the control group (p < 0.05).