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Figure 1 | Veterinary Research

Figure 1

From: Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis

Figure 1

Schematic depicting the experimental design used in the current study. MDM were cultured in 24-well tissue culture plates and infected with M. avium subsp. paratuberculosis (MOI 2:1). RNA was extracted from infected (MPTb) and non-infected control MDM at three time points post-infection: 2 h, 6 h and 24 h. In addition, RNA was extracted from 0 h non-infected control MDM to assess potential non-experimental changes in gene expression. The MDM lysates from replicate tissue culture wells (shaded) were pooled for RNA extraction. Global gene expression for the non-infected control and infected MDM was analysed using the Affymetrix® GeneChip® Bovine Genome Array.

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Veterinary Research

ISSN: 1297-9716