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Figure 4 | Veterinary Research

Figure 4

From: What have we learned from brucellosis in the mouse model?

Figure 4

Spleen cell population profiles and histopathology after infection of BALB/c mice with B. abortus 2308. (A) Spleen cell populations. The total number of CD4 T cells, CD8 T cells, neutrophils (PMN) and macrophages (Mø) per spleen was determined by multiplying the percentage of positive cells obtained by differential microscopy observation after cytospin centrifugation and fluorescent flow cytometry analysis by the total leukocyte count. Standard deviation at all points is lower than 10 % of the respective value (adapted from [57]). (B) Spleen histopathology and detection of Brucella antigens in the spleen. (a) Normal spleen (arrow points to the central artery). (b-f) Histological sections of spleen nodules during the acute phase of infection with virulent B. abortus 2308 (b) Spleen nodule with a clearer area infiltrated by macrophages (arrow points to the central artery). (c) Macrophage and histiocytes (arrow) infiltrating the spleen nodule. (d) Active extramedullary hematopoiesis (white arrow and insert) with granulomas (black arrow) in some areas of the spleen. (e) Immunoperoxidase detection of Brucella LPS antigen around the central artery of a spleen nodule. (f) Immunoperoxidase detection of Brucella LPS antigen within macrophages (arrow).

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