Figure 3From: Inability of NS1 protein from an H5N1 influenza virus to activate PI3K/Akt signaling pathway correlates to the enhanced virus replication upon PI3K inhibitionAnalysis of the interactions between NS1 proteins and p85β using yeast trap assays. A) AH109 yeasts transformed with the indicated plasmids were firstly plated onto DDO plates for 4 days of incubation at 30°C. And then, single AH109 colonies were picked and streaked on QDO/X-α-gal agar plate and incubated at 30°C for 3 days. The representative colonies were presented. (B) Different NS1-encoding plasmids were transformed into AH109 yeasts together with pGBK-p85β plasmid for α-galactosidase activities assays in liquid culture using PNP-α-gal as substrate. Data were from three independent experiments. BK-p53 + AD-T and BK-Lam + AD-T represented positive and negative control, respectively. Statistical comparison of different values was performed by the Student t test. Groups marked with asterisks are statistically different from the negative group (P < 0.01).Back to article page