Table 1 Solvent systems for TLC analysis of mycobacterial lipids (adapted from[22]).
Solvent System | Run Direction | Components | Runs | Lipids Resolved |
|---|---|---|---|---|
A | 1 | petroleum ether : ethyl acetate (98 : 2) | 3 | PDIM, TAG, MQ |
| Â | 2 | petroleum ether : acetone (98 : 2) | 1 | |
B | 1 | petroleum ether : acetone (92 : 8) | 3 | AT, FA |
| Â | 2 | toluene : acetone (95 : 5) | 1 | |
C | 1 | chloroform : methanol (96 : 4) | 1 | FA, GLY |
| Â | 2 | toluene : acetone (80 : 20) | 1 | |
D | 1 | chloroform : methanol : water (100 : 14 : 0.8) | 1 | CF, SL, DAT |
| Â | 2 | chloroform : acetone : methanol : water (50 : 60: 2.5 : 3) | 1 | |
E | 1 | chloroform : methanol : water (60 : 30 : 6) | 1 | DPG, PE PI, PIM |
| Â | 2 | chloroform : acetic acid : methanol : water (40 : 25 : 3 : 6) | 1 |