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Table 1 Bacterial strains and plasmids used in this study.

From: VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages

 

Description

Reference

STRAINS

  

H. parasuis

  

Nagasaki

virulent reference strain, serovar 5

[22]

PC4-6P

virulent field strain,serovar 12

[20]

SW114

non-virulent reference strain, serovar 3

[22]

F9

non-virulent field strain, serovar 6

[20]

E. coli

  

EPI300

Phage T1-resistant

Epicentre Biotechnologies

PLASMIDS

  

pCC1FOS

inducible copy, CmR

Epicentre Biotechnologies

pACYC184

low copy, CmR, TetR

ATCC number 37033

pEGFP

gfp, AmR

Clontech

pCC1FOS-8

pCC1FOS with a 47,195 bp insert, including vtaA8

this paper

pCC1FOS-9

pCC1FOS with a 38,659 bp insert, including vtaA9

this paper

pMCH-vtaA8

vtaA8 cloned in the BamHI site of pACYC184

this paper

pMCH-vtaA9

vtaA9 cloned in the BamHI site of pACYC184

this paper

  1. Cm: Chloramphenicol.
  2. Tet: Tetracycline.
  3. Am: Ampicillin.