From: VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages
Description | Reference | |
---|---|---|
STRAINS | ||
H. parasuis | ||
Nagasaki | virulent reference strain, serovar 5 | [22] |
PC4-6P | virulent field strain,serovar 12 | [20] |
SW114 | non-virulent reference strain, serovar 3 | [22] |
F9 | non-virulent field strain, serovar 6 | [20] |
E. coli | ||
EPI300 | Phage T1-resistant | Epicentre Biotechnologies |
PLASMIDS | ||
pCC1FOS | inducible copy, CmR | Epicentre Biotechnologies |
pACYC184 | low copy, CmR, TetR | ATCC number 37033 |
pEGFP | gfp, AmR | Clontech |
pCC1FOS-8 | pCC1FOS with a 47,195 bp insert, including vtaA8 | this paper |
pCC1FOS-9 | pCC1FOS with a 38,659 bp insert, including vtaA9 | this paper |
pMCH-vtaA8 | vtaA8 cloned in the BamHI site of pACYC184 | this paper |
pMCH-vtaA9 | vtaA9 cloned in the BamHI site of pACYC184 | this paper |