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Figure 4 | Veterinary Research

Figure 4

From: Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families

Figure 4

ODN 2216 induces an antiviral state both in stimulated PBMCs directly and in target cells incubated with supernatants from stimulated PBMCs. (A) mRNA expression factors of the indicated genes were measured in PBMCs of the individual cats (c01-c14) from four different age groups after stimulation with ODN 2216 for 24 h. (B) Mx mRNA expression factors were assessed at the indicated time points in PBMCs of one cat after a single stimulation with either ODN 2216 or ODN 2243. (C) Mx mRNA expression factors were measured in fcwf-4 cells incubated for 24 h with supernatants (Sup 2216, Sup 2243, Sup Neg) derived from PBMCs of eight adult cats (groups 2 and 3) or 100U recombinant feline IFNα (rfeIFNα). (D) Mx protein was detected by Western blot in fcwf-4 cells incubated with the indicated supernatants derived from PBMCs of one cat belonging to group 2 or with 100U recombinant feline IFNα (rfeIFNα) for 24 h. (E) Mx mRNA expression factors were measured in fcwf-4 cells at the indicated time points after a single stimulation with Sup 2216, Sup 2243 and Sup Neg respectively. (F) Mx protein was detected by Western blot in fcwf-4 cells at indicated time points after stimulation with Sup 2216 derived from PBMCs of the same cat (right panel). **p < 0.01. rfeIFNα = recombinant feline IFNα.

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