Figure 5

Infection of equine PBMC with recombinant viruses. PBMC were incubated with EHV-1 or EHV-4 at an MOI of 1 or 6 (a) for 1 h at 37°C. After 48 h, the percentage of infected cells was determined by flow cytometry. In another experiment, PBMC were infected with the engineered mutant viruses (b). The data represent the mean ± SD of at least three independent experiments.