Gene expression studies of host response to Salmonid alphavirus subtype 3 experimental infections in Atlantic salmon

Table 1 Primers and probe sequences used for quantitative real-time PCR

Genes		Primer sequence	GenBank accession no.
β-actin	Fwd	CCAGTCCTGCTCACTGAGGC	AF012125
	Rev	GGTCTCAAACATGATCTGGGTCA
SasaIFN-α	Fwd	TGGGAGGAGATATCACAAAGC	AY216594
	Rev	TCCCAGGTGACAGATTTCAT
Mx	Fwd	TGCAACCACAGAGGCTTTGAA	U66475
	Rev	GGCTTGGTCAGGATGCCTAAT
ISG15	Fwd	AAGTGATGGTGCTGATTACGG	AY926456
	Rev	TTGGCTTTGAACTGGGTTACA
IFN-γ	Fwd	CTAAAGAAGGACAACCGCAG	AY795563
	Rev	CACCGTTAGAGGGAGAAATG
TNF-α (1&2)	Fwd	AGGTTGGCTATGGAGGCTGT	NM_001123589
	Rev	TCTGCTTCAATGTATGGTGGG	NM_001123590
IL12-β	Fwd	CTGAATGAGGTGGACTGGTATG	BT049114
	Rev	ATCGTCCTGTTCCTCCG
IL-10	Fwd	CGCTATGGACAGCATCCT	EF165029
	Rev	AAGTGGTTGTTCTGCGTT
IL-8	Fwd	GGCCCTCCTGACCATTACT	NM_001140710
	Rev	ATGAGTCTACCAATTCGTCTGC
CD3-ε	Fwd	TCAGGGCTCGGAAGAAGTCT	NM_001123622
	Rev	GCCACGGCCTGCTGA
CD4	Fwd	GAGTACACCTGCGCTGTGGAAT	NM_001124539
	Rev	GGTTGACCTCCTGACCTACAAAGG
CD8-α	Fwd	CACTGAGAGAGACGGAAGACG	NM_001123583
	Rev	TTCAAAAACCTGCCATAAAGC
TCR-α	Fwd	GCCTGGCTACAGATTTCAGC	BT050114
	Rev	GGCAACCTGGCTGTAGTAGC
MHC I	Fwd	CTGCATTGAGTGGCTGAAGA	AF508864
	Rev	GGTGATCTTGTCCGTCTTTC
MHC II	Fwd	TCTCCAGTCTGCCCTTCACC	BT049430
	Rev	GAACACAGCAGGACCCACAC
NSAV-E2*	Fwd	CAGTGAAATTCGATAAGAAGTGCAA	EF675594
	Rev	TGGGAGTCGCTGGTAAAGGT
E2 Probe*		FAM-5’- AGCGCTGCCCAAGCGACCG- 3’-MGB

ISSN: 1297-9716