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Figure 3 | Veterinary Research

Figure 3

From: Two major ruminant acute phase proteins, haptoglobin and serum amyloid A, as serum biomarkers during active sheep scab infestation

Figure 3

Relationship between lesion area and serum Hp during a 6 week infestation of sheep with Psoroptes ovis. Panel a: Serum from a sheep infested with P. ovis in trial TCT where the lesion resolved naturally after 4 weeks (Sheep 1); Panel b: Serum from an infested sheep in the same trial where the lesion had continued to expand during the 6 week time course (Sheep 5). Immunoblots were probed with a rabbit polyclonal anti-human Hp antibody (Abcam) and the secondary antibody conjugate used was a swine anti-rabbit IgG HRP (Dako). The image was visualised by ECL Plus and ImageQuant. Band volumes (at 45 kDa) were quantified by ImageQuant TL software.

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