Figure 5

Quantitative analyses of serum acute phase protein and Pso o 2-specific IgG levels during an infestation/treatment/re-infestation model of Psoroptes ovis in sheep. Panel a: Haptoglobin: Sera samples from individual sheep (n = 12) in the PIT and SIT trial were analysed in duplicate using a colorimetric assay (ReactivLab). Hp concentrations are shown as mean (±SEM) through the time course of primary infestation (PIT) with P. ovis for 6 weeks, and post-treatment over 14 weeks, followed by secondary infestation (SIT) for a further 6 weeks and post-treatment for 2.5 weeks. Panel b: SAA: Sera samples from individual sheep (n = 12) in the PIT and SIT trial were analysed in duplicate using SAA ELISA (Tridelta Development Ltd). SAA concentrations are shown as mean (± SEM) through the time course of primary infestation (PIT) with P. ovis for 6 weeks, and post-treatment over 14 weeks, followed by secondary infestation (SIT) for a further 6 weeks and post-treatment for 2.5 weeks. Panel c: Pso o 2-specific IgG: Sera samples from individual sheep (n = 12) in the PIT and SIT trial were analysed in duplicate using an ELISA in which the coating antigen was a recombinant Pso o 2 (rPso o 2) at 75 μg/mL; primary antibody was IgG in the serum samples and the conjugate was a rabbit anti-sheep IgG HRP (Dako) at a dilution of 1:2000. IgG levels are represented as mean normalised OD₄₅₀ values (±SEM). *p ≤ 0.05; ** p ≤ 0.01 and ***p ≤ 0.001 as determined by repeated measures one-way ANOVA (Graph Pad Prism).