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Figure 2 | Veterinary Research

Figure 2

From: Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine

Figure 2

Genetic and phenotypic characterization of spontaneous Rev 1 R mutants. (A) Southern blot analysis of B. melitensis Rev 1 spontaneous R variants with IS711 (left panel), wbkA (center panel) and GI2 BMEI0999 (right panel) as probes. Note that, for the IS711 probe and B. melitensis Rev 1 and ∆wbkA but not ∆GI-2, the signal at 4.2 kb corresponds to two bands (predicted MW 4.2 and 4.14 kb). For simplicity, fragments with molecular masses lower than 3.8 kb were omitted. (B) Western blot of purified R-LPS and S-LPS, Rev 1, wbkA and GI-2 R mutants and the corresponding complemented strains probed with anti-LPS antibodies.

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