Table 1 Brucella strains used.
Strain | Characteristics | Source/ reference |
|---|---|---|
B. melitensis H38 | B. melitensis smooth virulent strain; S-LPS | CITA collection |
H38-NxR | B. melitensis H38 spontaneous mutant resistant to nalidixic acid; challenge studies in mice; S-LPS | This work |
Rev 1 | B. melitensis smooth attenuated reference vaccine strain; S-LPS | CITA collection |
Rev 1ΔwbkA | Rev 1 spontaneous deletion mutant in wbkA (ORF BMEI1398 and BME1412); R-LPS | This work |
Rev 1ΔGI-2 | Rev 1 spontaneous deletion mutant in GI-2; R-LPS | This work |
Rev 1ΔISBm1 | Rev 1 in-frame deletion mutant in ISBm-1 transposase gene (ORF BMEI1398 and BME1400); S-LPS | This work |
Rev 1Δint | Rev 1 in-frame deletion mutant in GI-2 phage-integrase gene (nucleotides intΔ53–286); S-LPS | This work |
Rev 2(ΔISBm1 Δint) | Rev 1 double in-frame deletion mutant in ISBm-1 transposase and GI-2 phage-integrase; S-LPS | This work |
Rev 1ΔwbkA/c | Rev 1ΔwbkA rough spontaneous mutant complemented with plasmid pMM14; S-LPS | This work |
Rev 1ΔGI-2/wboAB | Rev 1ΔGI-2 rough spontaneous mutant containing the plasmid pMM76; R-LPS | This work |
Rev 1ΔGI-2/c | Rev 1ΔGI-2 rough spontaneous mutant complemented with plasmid pBGI-997-99c; S-LPS | This work |