Skip to main content
Figure 3 | Veterinary Research

Figure 3

From: Characterization of NCR1+ cells residing in lymphoid tissues in the gut of lambs indicates that the majority are NK cells

Figure 3

NCR1+ cells in relation to CD3. (A) Flow cytometric plots of viable mononuclear cells from blood, distal jejunal lymph node (DJLN) and jejunal Peyer’s patch (JPP) of lambs. Cells were labeled with fixable Live/Dead (aqua) dye, followed by labeling for NCR1, then permeabilization and labeling for a conserved intracellular CD3 epitope (CytCD3). Plots are representative of five animals, at approx. 6 months of age. (B-G) Representative figures of 5 one month old lambs, showing the in situ distribution of NCR1+/CD3- cells and T cells in jejunal Peyer’s patch (B), ileal Peyer’s patch (C), colon patch (D), jejunum (E), superficial cervical lymph node (F) and tonsil (G). A two-colour fluorescent labeling with the NK cell antibody NCR1 (green) and the pan T cell marker CD3 (red) was used. The NCR1+/CD3- cells were present at a moderate to large amount in the compartments of dome (d), interfollicular area (i), at a moderate amount in lamina propria (lp), while very few NCR1+ cells were found in the follicle (f). In the follicle-associated epithelium (fae), NCR1+/CD3- cells were observed intermittently (arrow). NCR1+/CD3+ cells were not observed, but NK cells and T cells were localised in the same areas, and were often seen in close contact with each other (inset; merged membranes appear yellow). To illustrate autofluorescence, photos were taken with a blue filter which did not detect signals from the secondary antibodies used in the study. The basal lamina in dome is delineated (B). Absorptive epithelium (ae); epithelium (ep); vessel (v). Bars 50 μm (B-G).

Back to article page