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Figure 3 | Veterinary Research

Figure 3

From: CD27 expression discriminates porcine T helper cells with functionally distinct properties

Figure 3

IFN-γ production of porcine T-helper cell subpopulations. Intracellular cytokine staining for IFN-γ of PBMCs stimulated with PMA/Ionomycin (left panel), or re-stimulated with PRRSV (middle panel) or FLUAVsw (right panel) was performed by six-colour FCM including a live/death discrimination dye. PBMCs re-stimulated with PRRSV or FLUAVsw were derived from animals that had been vaccinated or infected with the respective virus. Cells cultivated in Medium or Mock served as negative controls. CD4+ T helper cells, gated in contour plots (black gates), are shown in dot plots for CD27 (x-axes) versus CD8α and CD45RC expression (y-axes) in light grey. CD4+IFN-γ+ T helper cells (contour plots; red gates) are highlighted in the respective dot plots in red. Numbers indicate percentage of IFN-γ producing cells within CD4+ T helper cells. Data of one representative animal is shown for each stimulation regimen (PMA/Ionomycin: 6 animals tested, PRRSV and FLUAVsw: 3 animals tested for each virus). For PMA/Ionomycin and FLUAVsw at least 3 × 105 lymphocytes and for PRRSV at least 8 × 105 lymphocytes per sample were acquired.

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