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Table 2 Gene-specific oligonucleotide primers used for qPCR

From: Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system

Gene symbol

Oligonucleotides (5′-3′)

Amplicon

Annealing

Accession

F: forward ; R: reverse

(bp)

Temperature (°C)

number (GenBank)

18 s rRNA

F: CGGGGAGGTAGTGACGAAA

196

69

AF176811

 

R: CCGCTCCCAAGATCCAACTA

   

TNFa

F:TCTTCTCAAGCCTCAAGTAACAAGC

104

69

EU276079

 

R: CCATGAGGGCATTGGCATAC

   

Il6

F: TGCTGGTCTTCTGGAGTATC

153

62

EU276071

 

R: GTGGCTGGAGTGGTTATTAG

   

Ccl2

F: GCTCGCTCAGCCAGATGCAA

117

62

NM174006

 

R: GGACACTTGCTGCTGGTGACTC

   

Ccl5

F: CTGCCTTCGCTGTCCTCCTGATG

217

62

NM175827

 

R: TTCTCTGGGTTGGCGCACACCTG

   

Ccl20

F: TTCGACTGCTGTCTCCGATA

172

62

NM174263

 

R: GCACAACTTGTTTCACCCACT

   

CD83

F: GAA GGG CAG AGA AAC CTG AC

231

65°C

BC112861

 

R : AGA GGT GAC TGG GAG GAA AG

   

Nos2

F: CTT GAG CGA GTG GTG GAT GG

240

64

NM001076799

 

R: CCT TCA TCC TGG ACG TGG TTC

   

Isg15

F: CGC-CCA-GAA-GAT-CAA-TGT-GC

  

NM_174366

 

R: TCC-TCA-CCA-GGA-TGG-AGA-TG

158

62

 

Cxcl10

F: TTC-AGG-CAG-TCT-GAG-CCT-AC

218

62

NM_001046551

 

R: ACG-TGG-GCA-GGA-TTG-ACT-TG

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