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Figure 1 | Veterinary Research

Figure 1

From: Immune gene expression profiling of Proliferative Kidney Disease in rainbow trout Oncorhynchus mykiss reveals a dominance of anti-inflammatory, antibody and T helper cell-like activities

Figure 1

Detection of T . bryosalmonae in rainbow trout kidney during clinical PKD. ( A ) T. bryosalmonae 18S rDNA relative to trout MCSF (DNA detection); ( B ) T. bryosalmonae RPL18 relative to trout EF-1α (mRNA detection). Average cCq value in grade 1 fish is provided above the bar; ( C ) Correlation between 18S rDNA and RPL18 parasite detection markers is shown (r = 0.94; P < 0.0001); ( D ) Linear relationship of RT-qPCR detection and molar concentration of T. bryosalmonae RPL18. A serial diluted RPL18 internal reference was included in the same PCR run to enable relative quantification.

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