Figure 5From: Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasmaInteraction of M. suis with porcine endothelial cells induces actin condensation in vitro. Confocal laser scanning micrographs of PAECs incubated with M. suis (1 × 104 cells/mL). Cytoskeletal actin was stained with FITC-phalloidin (green); M. suis cells were stained with anti-HspA1 antiserum and TRITC-labeled secondary antibodies (red). Nuclei and bacteria were counterstained with DAPI (blue). The image represents an overlay of all fluorescent channels. (A) 90 min post-infection (pi); scale bar = 40 μm; (B) 6 hours pi; scale bar = 20 μm; (C) 4 days pi; scale bar = 10 μm; (D) Pre-infection; scale bar = 50 μm.Back to article page