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Figure 2 | Veterinary Research

Figure 2

From: BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals

Figure 2

BAG3 expression and down-modulation in equine and human cell lines. (a) BAG3 expression in equine cell lines. EqS04b, E. Derm and human HeLa cell total extracts were analysed by Western blotting for BAG3 levels. The blots were also probed for tubulin as the loading control. Blot is from one experiment representative of at least two with similar results. On the right: densitometry analysis of bands. (b) BAG3 down-modulation in EqS04b and E. Derm cells. EqS04b and E. Derm cells were transfected with BAG3siRNA (siRNA) or a non-targeting siRNA (scrRNA). Non-transfected cells were included as controls (Ctrl). At the times indicated, total cell extracts were prepared and analysed by Western blotting for BAG3 levels. The blots were also probed for tubulin as the loading control. Blots are from one experiment representative of at least two with similar results.

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