From: Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae
PCR product | Size (bp) | Restriction site | Oligonucleotide sequence (5′ - 3′) |
---|---|---|---|
M. hyopneumoniae strain 232 oriC- i | 2130 | Nco I | F - AGGCCATGG TTGTTAATTATTGCTTGAAATTC |
Spe I | R - AGTACTAGT AAACTTTATAGGAAAGTTCG | ||
M. hyopneumoniae strain 232 oriC- ii | 1823 | Nco I | F - GATGCCATGG TTGGTTGATAAGTTCGCTTC |
Spe I | R - GTAGACTAGT CATTCTTTCAATTTGTCATTC | ||
M. hyopneumoniae strain 232 oriC- iii | 859 | Nco I | F - GATGCCATGG TTGGTTGATAAGTTCGCTTC |
Spe I | R - GCTCACTAGT GACAATCAGTTTAAAAATTCAC | ||
M. hyopneumoniae strain 232 oriC- iii | 859 | Aat II | F - GCAGGACGTC TTGGTTGATAAGTTCGCTCC |
Nco I | R - GACGCCATGG GACAATCAGTTTAAAAATTCAC | ||
M. hyopneumoniae strain 232 oriC- iv | 422 | Nco I | F - GCATCCATGG ATATTGTGCATGCCCGCGGATAT |
Spe I | R - GTAGACTAGT CATTCTTTCAATTTGTCATTC | ||
M. hyopneumoniae strain 232 oriC- v | 280 | Aat II | F - GCAGGACGTC TTGGTTGATAAGTTCGCTCC |
Nco I | R - CGTACCATGG CCTAATTTGCTGTGTTCTAA | ||
tetM + spiralin promoter from plasmid pSRT2 | 2320 | Pst I | F - TAACTGCAG CAAAAGCTTGCATGCCTGCA |
Spe I | R - GTCACTAGT CAGTTCAGATCTTTATATAAC | ||
tetM from plasmid pIVT | 2109 | Spe I | F - ATCTACTAGT CATGTGATTCTAAAGTATCC |
Pst I | R - GATCTGCAG CTTATTTAACAAGAAACC | ||
tetM (without promoter) from plasmid pSRT2 | 1963 | Bam HI/Pst I | F - GAACTGCAGGATCC ATGGAGGAAAATCACATGA |
Sal I | R - CCCGGTCGACTTATATAACAACTTAAATTAC | ||
M. hyopneumoniae strain 232 P97 promoter | 619 | Bam HI | F - GAATGGATCCCCAACAATTCCGGCAGTC |
Spe I | R - GCTAACTAGT ACGGGGATTTAAAACAGAAAC | ||
M. hyopneumoniae strain 232 ldh promoter | 421 | Spe I | F - GGCTAACTAGT ATAGAATTTTGCAATTAAAG |
Bam HI | R - CTTAGGATCC ATTTATACTCGTATTTGTTAT | ||
M. hyorhinis oriC | 1479 | Nco I | F - GACTCCATGG TCGGAATCGAGATTCCTAATT |
Spe I | R - GCCTACTAGT CTACACCTTCGATTTCTCTAA | ||
M. pulmonis oriC | 1909 | Nco I | F - ATTAGCCATGG CACTCTGGTCAGCGCTAGATC |
Nhe I | R - TCGCGCTAGC CTAACTTGAAAATAAGCTCC | ||
M. conjunctivae oriC | 2307 | Nco I | F - GATACCATGG TATGCCTTTGGTCTAGGATG |
Xba I | R - CGATGTCTAGA CCCTTGAACAAATATACCA | ||
M. ovipneumoniae oriC | 2102 | Nco I | F – AGTCCATGGTCAATTATTGCTTGGAATTC |
Spe I | R - CGTACTAGTAAACTTTATAAGAAAGTTCGCT | ||
bla gene (DIG-labelled probe) | 860 | N/A | F – CCAATGCTTAATCAGTGAGG |
R - GTATGAGTATTCAACATTTCCG | |||
tetM (DIG-labelled probe) | 406 | N/A | F – GTGGACAAAGGTACAACGAG |
R - CGGTAAAGTTCGTCACACAC |