Figure 2
From: Multiple introductions of serotype O foot-and-mouth disease viruses into East Asia in 2010–2011
Deletion in the S-fragment of the genome of some FMDVs belonging to the O/SEA/Mya-98 lineage. a) Sequence alignment showing the deletion in the genome of some FMDVs belonging to the O/SEA/Mya- (70 nt length versus viruses from the same region and year) and other FMDV genomes available in GenBank. Sequences represented by names in lower case are derived from cell culture isolates generated from field strains (C3/Resende/BRA/1955, C/UK/149/1934, A/Canefa/ARG/1/1961; A25/BA/Argentina/1959 and A2/Spain/43); b) Representative results for RT-PCR amplification of the S-fragment of O/HKN/20/2010 and other related viruses from Hong Kong SAR; A) The use of the primer pairs O1F/O1R amplified two different products of approximately 310 and 380 bp depending on the presence or absence of the deletion, respectively. B) The amplification of the S-fragment of those viruses with a deletion failed when replacing the forward primer for a new primer (O1F2: 5'-ACC GAC TAG TAC TCT TAA CAC TCC GC-3′) targeting this specific region.