Figure 2
Changes in levels of mRNAs for porcine CC ligands following infection of pigs with different ASFV isolates. Changes in mRNA for chemokine and chemokine receptor genes in porcine blood cells were estimated by real time RT PCR and are shown for samples from experiment 2. Pigs were either uninfected or infected with ASFV isolates, OURT88/3 or Benin97/1. Samples were taken at 0 3, 7, 14 or 20 dpi and RNA was extracted. mRNA levels were measured by real time RT-PCR using SYBR Green (Sigma). Changes are shown as Log2 fold changes (y-axis) compared to 2 time points pre-infection to give the calibrator sample. No amplification was detected for CCL2 mRNA in calibrator samples so data shown is the Ct value. Data from real time RT PCR for (A) CCL2, (B) CCL3L, (C) CCL4, (D) CCL5, (E) CCR1, (F) CCR5, (G) CXCL10, are shown. The dpi is shown on the x axis and the different isolates are indicated on the right of each panel. The mean for each group ± SEM is shown, n = 3-6 pigs.