Figure 5

Immunoperoxidase staining of HO-1 in the lung and small intestine of T . gondii infected mice. BALB/c and C57BL/6 mice were orally infected with 5 T. gondii cysts and treated with ZnPPIX (10 mg/kg/day), hemin (5 mg/kg/day) or vehicle (Na₂CO₃ + NaCl) and analyzed at day 12 pi. Representative photomicrographs of lung of hemin-treated mice (a) and small intestine of untreated/uninfected mice (c). The images were captured using a 10 × objective. The data were obtained by counting the number of HO-1 positive cells per tissue section of the lung (b) and small intestine (d) using a 40 × objective. Data represent the median with interquartile range of each experimental condition in two independent experiments. ^*Statistically significant differences between the experimental conditions within the same mouse lineage (P < 0.05, Kruskal Wallis test and Dunn multiple comparison post-test); ^#Statistically significant differences between the two mouse lineages (P < 0.05, Mann–Whitney test).