Figure 4From: H3N2 canine influenza virus causes severe morbidity in dogs with induction of genes related to inflammation and apoptosisHistopathology and immunohistochemistry of the lungs of infected dogs. The left cranial lobes of the lungs from dogs (Korean mongrel, n = 2) infected with A/Korea/S1/Canine/2012 (H3N2) and from PBS mock-infected dogs were collected 5 days after infection. The lung tissues from PBS mock-infected (A) or infected (B) dogs were stained with H&E stain. In addition, the lung tissues from PBS mock-infected (C) or infected (D) dogs were stained with mouse anti-influenza A nucleoprotein antibody, biotin-labeled goat anti-mouse immunoglobulin, and red alkaline phosphatase substrate. The stained sections were imaged with an Olympus DP70 microscope.Back to article page