Figure 5

Microarray and real-time PCR analysis of genes associated with Toll-like receptors, NK cells, macrophages, and neutrophils. The mRNA from the left cranial lobes of the lungs from dogs (Korean mongrel, n = 3) infected with A/Korea/S1/Canine/2012 (H3N2) and from PBS mock-infected dogs were subjected to microarray analysis. The genes induced higher in the infected dogs were quantified by real-time PCR. A, Heat map of genes related to TLR; B, Heat map of genes related to NK cells; C, The fold-change comparison of genes related to NK cells; D, Heat map of genes related to macrophages; E, Microarray data of Toll-like receptors, NK cells, macrophages, and neutrophils; F, mRNA fold changes of selected genes of Toll-like receptors, NK cells, macrophages, and neutrophils. *P value < 0.05, ** P value < 0.01.