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Table 2 List of the 20 most significant upstream regulators identified as involved in gene regulation after vaccination as determined by IPA analysis.

From: Monitoring the immune response to vaccination with an inactivated vaccine associated to bovine neonatal pancytopenia by deep sequencing transcriptome analysis in cattle

Upstream regulator

Molecule type

Predicted activation state1

p-value of overlap

Number of target molecules in dataset

miR-30c-5p (and other miRNAs w/seed GUAAACA)

mature microRNA

Inhibited

1.75E-07

29

MYCN

transcription regulator

Inhibited

4.08E-07

52

TCR

complex

 

1.02E-06

57

miR-155-5p (miRNAs w/seed UAAUGCU)

mature microRNA

Inhibited

1.97E-06

34

CD40LG

cytokine

Activated

2.06E-06

67

CD28

transmembrane receptor

Inhibited

6.13E-06

62

mir-210

microRNA

 

1.58E-05

16

E2F1

transcription regulator

Activated

8.88E-05

52

miR-124-3p (and other miRNAs w/seed AAGGCAC)

mature microRNA

Inhibited

1.23E-04

38

HOXA9

transcription regulator

 

1.33E-04

34

IL2

cytokine

Activated

1.77E-04

58

CD3

complex

Inhibited

4.26E-04

98

miR-291a-3p (and other miRNAs w/seed AAGUGCU)

mature microRNA

Inhibited

4.55E-04

18

SMARCC1

transcription regulator

 

7.17E-04

4

YWHAQ

other

 

7.37E-04

5

SHOX

transcription regulator

 

7.37E-04

5

TGFB1

growth factor

Activated

7.99E-04

187

FSH

complex

 

1.64E-03

60

TMBIM6

other

 

1.68E-03

6

miR-17-5p (and other miRNAs w/seed AAAGUGC)

mature microRNA

Inhibited

1.94E-03

10

  1. 1 For identification of activation or inhibition of upstream regulators, a threshold for the activation z-score calculated in the IPA analysis of |z-score| > 2 was applied.