Figure 1

Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203a^hi leukocytes (d) and marked with blue color. Monocytes were gated as CD203a^low/- SWC8^-(e) CD172a^hi(f) leukocytes where the CD203a^low/- region was defined as the complementary region to the CD203a^hi region. Then, SLA-DR⁺ monocytes were marked with red color and SLA-DR^- monocytes were marked with green color (g). SLA-DR^- region was defined as the complementary region to the SLA-DR⁺ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR^–, red: SLA-DR⁺) in various body compartments of control and APP-infected pigs.