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Table 1 Primers for chemokines, chemokine receptors, CD62L and reference gene used in quantitative real-time PCR.

From: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

Gene

Alternative name

Primer forward / reverse

Tm

Length of product

CCL1

I-309

CCGTGGTTCTGGTGTGCCTGCTG / ACATGCATGCTCTTGCTGTCCACCT

68.19/67.01

70

CCL2

MCP-1

CCGAAGCTTGAATCCTCATC / TAGCAGCAGGTGACTGGAGA

56.30/60.25

134

CCL3-like1

MIP-1αS

TTTTGAGACCAGCAGCCAGT / TCAGCTCCAGGTCAGAGATG

59.82/58.52

122

CCL4

MIP-1β

GCTCCCAGCCAGCCGTGGTATTC / CAGTCATCACTGGGGTTGGCGCA

67.64/67.50

70

CCL5

RANTES

ACCACACCCTGCTGTTTTTC / GGCGGTTCTTTCTGGTGATA

58.89/57.60

124

CCL8

MCP-2

CAGTGTCCCCAGGAGGCTGTGATCTTC / GTCCGCGCAGACCTCCTTGTCG

68.18/67.74

60

CCL11

Eotaxin

TCCCCAGAATGCTGTGATCTTCAGCAC / GGTCCAGGTACTCCATGGCATTCTGAACC

66.79/68.35

98

CCL17

TARC

CTCCTCCTGGGGGCTTCCCTGC / CAGCACTCCCGGCCCACGTTG

68.64/68.37

68

CCL19

ELC

CCACGCTGAAAGGTCGCGAGCTC / TTGCGGTGGTGCTTGCTCTTAGC

68.18/66.13

106

CCL20

MIP-3α

TGCAGCAAGTCAGAAGCAGCAAGC / CGAGCTGCTGTGTGAAGCCCATG

66.60/66.44

100

CCL21

SLC

CCCCCACACCCAAGGCAGTGATG / AACCCAGGCTTGGCTCCTGCTTC

67.60/67.31

116

CCL22

MDC

TCAGACTCCTGCCGGAGGCCTG / CAGGGCAGTCTGGGGTCAGCAC

67.96/67.30

80

CCL25

TECK

TTCTTCCAAGGCCCTCAGTCTGGAGTGA / ACTGTGGGCTCATGGTCCTGGAATAGC

68.56/68.05

147

CCL27

CTACK

CCAGGCCTTCGTGCTTCACCTGTC / TCCCCTGGAGCCTCCTTCCATGG

67.69/67.54

104

CCL28

MEC

GAAGCTATACTTCCCATTGCCTCCAGCT / TGCGCTTGACATGAAGGATGACAGCA

66.27/67.17

148

CXCL9

Mig

AGCAGTGTTGCCTTGCTTTTGGGTATCATC / GCTGGTGTTGATGCAGGAACAACGTCC

67.76/68.40

102

CXCL10

IP-10

CCCACATGTTGAGATCATTGC / CATCCTTATCAGTAGTGCCG

57.57/55.44

168

CXCL11

I-TAC

ACAAAACAGAAGTGATTGTCACCCTGAAAGCAC / GCTTGCTTTGATTTGGGATTTAGGCATCTTCGT

67.68/67.90

73

CXCL12

SDF-1α/β

CCTCGTGCTGACCGCGCTCTG / CGAGAGAGTGGGACTGGGTTTGTTTAAAGCT

68.06/68.07

256

CXCL14

BRAK

CGGCCAGCATGAGGCTCCTGAC / ACTTGCATTTGGACCCGTCCACGC

67.66/68.16

87

CXCL16

SR-PSOX

TCGCGGAGAATGTGGACGTGCTC / TCGTCTGGGCAGGGGTGCTACTG

67.20/68.13

128

CX3CL1

Fractalkine-like

GGCTGCTGGCCTTCCTTGGTCTC / GGGAGCAGCCCTGCAGACTCTGG

67.59/68.97

78

CCR1

 

GCTCCAGAAACAAAGACTTCGTGGACACAG / ATGTTGCTGCTAAAGGCAGTGGGCT

67.58/67.26

70

CCR2

 

TGCCACTTGGAAGAAGCAACAGACCG / CAGAGAGTGAGATGTGGGCAGCACG

67.54/67.21

100

CCR3

 

TCCCCATCAACGGGAGAGCAGGA / CTTCAGAGCTTCAGTGCTGTGCTGG

67.16/65.93

137

CCR4

 

GCCATTGTGCACGCGGTCTTCTC / GTGAAGCGAACACAGCCACCGAC

66.91/66.55

97

CCR5

 

TGGTCAGAGGAGCTGAGACA / AGAAGGGACTCGTCGTTTGA

59.89/58.67

86

CCR6

 

AGCGAGAGGGCGCTGGGCTAC / GTTGAGGCAGCAGTGCAGGAACG

68.88/66.60

69

CCR7

 

GTGGTGGCTCTCCTTGTCAT / GAAGCACACGGACTCGTACA

59.67/60.04

114

CCR9

 

CCACAGAAGCCGCAAGTCTGATGC / TGGCTTGCAAACTGCCTGACATGGT

66.45/67.64

139

CCR10

 

CTGCAGCTGCCCTACAGTCTCGC / AGATCCTTGCGCTTGCTGGCAGG

67.54/67.85

95

CXCR3

 

GTAGGGTGGACGTAGCCAAG / GGAACTTGACACCCACGAAG

59.82/58.77

96

CXCR4

 

TACCATGGACGGGTTCCGTATATTCACTTCAG / GCATTTTCCTCCCGGAAACAGGGTTCC

67.26/67.89

108

CXCR6

 

CCACTGACAGAGCACCTCTACTGGGTC / CCCCTGGCTGCTGTCGTTGGAAG

67.67/67.77

106

CX3CR1

 

CCAGTCACCTGCTGGCAACCCTACC / GGAAGCTGGTTTTGGGCTCTGGCTC

68.98/67.96

88

CD62L

 

AGCTTCTTGTCAGCCCAGGTCATGC / CCCAGAGGCTCACACTGAGTCACGAAC

67.25/68.46

132

CD163

 

CTTGGGGCAGCGTTGGCAGGAATAG / ATGCAGGGCTGATGTCCCCTCTGTC

68.02/68.16

99

SLA-DR

 

TGCTTGGAGTGTCACCATCTG / GGTCTGCTCTTTGTTGCTGTG

60.27/60.00

115

TBP1

 

AACAGTTCAGTAGTTATGAGCCAGA / AGATGTTCTCAAACGCTTCG

59.75/56.48

153

HPRT

 

GAGCTACTGTAATGACCAGTCAACG / CCAGTGTCAATTATATCTTCAACAATCAA

61.41/58.88

111