Veterinary Research

Table 4 Chemokine receptor, CD62L, CD163 and TBP1 expression by bone marrow and peripheral blood CD163 ⁺ monocytes from control and APP-infected pigs.

From: Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines

	Bone marrow						Peripheral blood
	Control			APP-infected			Control			APP-infected
CCR1	0.87	±	0.12 *	0.47	±	0.07 *	0.87	±	0.13	1.09	±	0.30
CCR2	0.35	±	0.07 *	0.18	±	0.02 *	0.23	±	0.08	0.11	±	0.03
CCR3	4.42	±	1.00 *†	1.86	±	0.62 *‡	18.79	±	4.77 †	9.21	±	4.28 ‡
CCR4	3.24	±	0.71 *†	1.33	±	0.41 *‡	13.44	±	3.25 †	6.42	±	3.00 ‡
CCR5	0.16	±	0.03 *	0.06	±	0.01 *‡	0.30	±	0.05	0.15	±	0.05 ‡
CCR6	n.d.			n.d.			n.d.			n.d.
CCR7	0.46	±	0.12 †	0.26	±	0.05	0.88	±	0.17 ⁰†	0.24	±	0.10 ⁰
CCR9	0.003	±	0.001	0.001	±	0.000	0.006	±	0.002 ⁰	0.001	±	0.000 ⁰
CCR10	2.62	±	0.54 *†	1.09	±	0.31 *‡	10.54	±	2.26 ⁰†	4.86	±	2.00 ⁰‡
CXCR3	2.55	±	0.55 *†	1.00	±	0.29 *‡	10.83	±	2.58 †	5.10	±	2.27 ‡
CXCR4	3.81	±	0.38 *†	2.04	±	0.15 *‡	8.70	±	0.68 †	9.05	±	1.82 ‡
CXCR6	0.002	±	0.001 *†	0.000	±	0.000 *	0.019	±	0.008 ⁰†	0.001	±	0.000 ⁰
CX3CR1	2.96	±	0.63 †	1.18	±	0.31 ‡	13.01	±	3.05 †	6.33	±	2.33 ‡
CD62L	0.81	±	0.20	0.86	±	0.21	0.77	±	0.32	1.29	±	0.27
CD163	5.31	±	0.52 *†	9.08	±	*2.27 ‡**	11.98	±	2.55 †	19.40	±	2.43 ‡
SLA-DR	16.62	±	2.77 *†	4.43	±	0.76 *	40.60	±	4.04 ⁰†	4.23	±	1.23 ⁰
TBP1	0.06	±	0.01	0.06	±	0.01	0.09	±	0.02	0.11	±	0.02

The data are shown as a fold change of chemokine receptor, CD62L, CD163, SLA-DR and TBP1 reference gene mRNA expression in relation to the reference gene HPRT as measured by the RT-qPCR. Data are expressed as mean ± S.E.M. (n = 5). The significant differences (Mann-Whitey U test, P < 0.05,) between control and APP-infected pigs are marked with * (bone marrow) or ⁰ (peripheral blood). The significant differences (Mann–Whitney U test, P < 0.05) between bone marrow and peripheral blood monocytes are marked with † (control) or ‡ (APP-infected). The values which were elevated between control and APP-infected pigs are highlighted in bold. N.d, non-detected.

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