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Figure 5 | Veterinary Research

Figure 5

From: Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate immune responses

Figure 5

Silencing of TLR15 decreases iNOS gene expression and NO production in MDLP stimulated HD11 macrophages. A) Silencing of TLR15 decreases iNOS gene expression. HD11 cells were transfected with siRNA specific for TLR15 (TLR15 siRNA) or scrambled negative control siRNA (NC siRNA) for 8 h and then stimulated with NAP or MDLP for additional 12 h. mRNA expression for iNOS and TLR15 was determined by RT-qPCR. Bars show mean ± S.E. (n = 4); **, p < 0.01 and ***, p < 0.001. B) Silencing of TLR15 decreases MDLP-induced NO production. HD11 cells were transfected with TLR15 siRNA or NC siRNA for 8 h and then stimulated with NAP or MDLP for additional 12 h. After, production of nitrite was measured in cell culture supernatants by Griess assay. Bars show mean ± S.E. (n = 4); ** p < 0.01.

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