Contribution of mammary epithelial cells to the immune response during early stages of a bacterial infection to Staphylococcus aureus

Brenaut, Pauline; Lefèvre, Lucas; Rau, Andrea; Laloë, Denis; Pisoni, Giuliano; Moroni, Paolo; Bevilacqua, Claudia; Martin, Patrice

doi:10.1186/1297-9716-45-16

Veterinary Research

Table 1 Primer sequences used for qPCR experiments

From: Contribution of mammary epithelial cells to the immune response during early stages of a bacterial infection to Staphylococcus aureus

Genes	Primers	Sequence 5′ > 3′	Amplicon size, nt
α_s2-casein (CSN1S2)	forward	CTG GTT ATG GTT GGA CTG GAA AA	76
	reverse	AAC ATG CTG GTT GTA TGA AGT AAA GTG
κ-casein (CSN3)	forward	AGG TGC AAT GAT GAA GAG TTT TTT C	66
	reverse	CCC AAA AAT GGC AGG GTT AA
Cluster of differentiation 3 epsilon (CD3e)	forward	ACG CTG TAC CTG AAA GCA AGA	118
	reverse	AAT ACA CCA GCA GCA GCA AG
Cluster of differentiation 68 (CD68)	forward	GAT CTG CTC TCC CTG AAG CTA CA	79
	reverse	CAT TGG GAC AAG AGA AAC TTG GT
Cluster of differentiation 18 (CD18)	forward	AGC GAC CTC AGG GAG TAC CA	65
	reverse	TTA TCG TTG TTC CAC TGG GAC TT
Chemokine 4 (CCL4)	forward	CAG CCG TGG TAT TCC AGA CC	109
	reverse	CTC GGA GCA GCT CAG TTC AGT
Cyclophilin A (PPAI)	forward	TGA CTT CAC ACG CCA TAA TGG T	62
	reverse	CAT CAT CAA ATT TCT CGC CAT AGA
Galectin 3	forward	GTG GTA AAC CTT TCA AAA TAC AAG TGC	101
	reverse	ATT TTT CAC CCG ATG ATT GTA CTG
G protein-coupled receptor 97 (GPR97)	forward	GAG ATC ACC TTC TCC CAC CAG	204
	reverse	TGT GGA GCA GCC CAA GGA
Interleukin 8 (IL-8)	forward	TGA GAG TGG GCC ACA CTG C	103
	reverse	CAC AAC CTT CTG CAC CCA CTT
Keratin 14 (KRT14)	forward	CCC AGC TCA GCA TGA AAG C	57
	reverse	AGC GGC CTT TGG TCT CTT C
Serum amyloid A3 (SAA3)	forward	CTG GGC TGC TAA AGT GAT CAG TAA C	69
	reverse	CCC TTG AGC AGA GGG TCT GTG ATT
S100A12	forward	TCC ACC AGT ACT CCA TCC GG	102
	reverse	TGG TGT TTT TGA GGC AGT TGG
Serglycin	forward	TCC AGC AGA ATC CCA CCT CTA	107
	reverse	CCA GAA CCT GAT CCT GAG ACG
SerpinA1	forward	AAG AAA TAT GCA AGT TCT GCC AAT T	101
	reverse	ACC CTG TTG ATG CCC AGT TC
TNFα	forward	CAG AGG GAA GAG CAG TCC CC	101
	reverse	TGG GCT ACC GGC TTG TTA TTT
Toll-like receptor 2 (TLR2)	forward	TAA ACT TGA GAG TGG AGG TCA AAT CA	101
	reverse	TCA GAG GCT CCT TCC GTG G
Interleukin 1beta (IL-1β)	forward	GAC AAC AAG ATT CCT GTG GCC	101
	reverse	TCT ACT TCC TCC AGA TGA AGT GT
Pentraxin 3 (PTX3)	forward	CCG AGC TGT GCA GGG CT	101
	reverse	GCA CGC TTG CAA AAA TCT TCT T
Cathelicidin	forward	GAG AAT GGG CTG GTG AAA CAG	107
	reverse	GGG CGA AGT CTC CTC ACA CTC
24S ribosomal protein (RPS24)	forward	TGG TGGTGG CAA GAC AAC TG	66
	reverse	TTC TTC GCG TAA TCC AAG GAA

Each pair of primers amplifies the target cDNA (amplicon sizes ranging between 57 and 248 nucleotides) in its 3′ UTR. Primer pairs were designed with primer Express Software v2.0 (Applied Biosystems) except for 24S ribosomal protein primers, which were manually designed.

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ISSN: 1297-9716

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